<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Bovine postpartum metritis causes great losses. Mast cell (MC)-released mediators participate in uterine inflammation and immune response, but their role in postpartum metritis in cows has not been reported. This study investigated the effect of endometrial MC on the disorder.<bold>Material and Methods:</bold> Ten dairy cows, at 6 to 10 days postpartum and with acute purulent metritis made up the experimental group, and 10 comparable healthy cows the control group. Endometrial histamine and IgE levels were determined by ELISA, and the MC particle state and expression of histamine H1 (H1R) and H2 (H2R) mRNA receptors were examined by transmission electron microscope and real-time quantitative PCR, respectively.<bold>Results:</bold> Endometrial histamine and IgE levels were significantly higher in the experimental group. In the control group, homogenously distributed size-varied granules were seen in MC cytoplasm of endometrium of lamina propria. In the experimental group however, these showed degranulation with features of reduction. The level of H1R mRNA was lower in the experimental group, but that of H2R mRNA was higher.<bold>Conclusion:</bold> The results suggest MC type I hypersensitivity characteristics during metritis, and histamine provocation of local inflammation. High expression of H2R and low expression of H1R inhibited the inflammatory response and prevented excessive uterine tissue damage.</abstract>

Introduction: Bovine postpartum metritis causes great losses. Mast cell (MC)-released mediators participate in uterine inflammation and immune response, but their role in postpartum metritis in cows has not been reported. This study investigated the effect of endometrial MC on the disorder.Material and Methods: Ten dairy cows, at 6 to 10 days postpartum and with acute purulent metritis made up the experimental group, and 10 comparable healthy cows the control group. Endometrial histamine and IgE levels were determined by ELISA, and the MC particle state and expression of histamine H1 (H1R) and H2 (H2R) mRNA receptors were examined by transmission electron microscope and real-time quantitative PCR, respectively.Results: Endometrial histamine and IgE levels were significantly higher in the experimental group. In the control group, homogenously distributed size-varied granules were seen in MC cytoplasm of endometrium of lamina propria. In the experimental group however, these showed degranulation with features of reduction. The level of H1R mRNA was lower in the experimental group, but that of H2R mRNA was higher.Conclusion: The results suggest MC type I hypersensitivity characteristics during metritis, and histamine provocation of local inflammation. High expression of H2R and low expression of H1R inhibited the inflammatory response and prevented excessive uterine tissue damage.

Role of mast cells in cow metritis

Institute of Biotechnology, Northeast University, Shenyang 110004, China

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Role of mast cells in cow metritis"}

Introduction: Bovine postpartum metritis causes great losses. Mast cell (MC)-released mediators participate in uterine inflammation and immune response, but...

Role of mast cells in cow metritis

Published Online: May 28, 2016

Page range: 177 - 180

Received: Oct 16, 2015

Accepted: May 16, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0026

Keywords
cow, metritis, mast cell, histamine

© 2016 Guo-Qing Wang et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Role of mast cells in cow metritis

Published Online: May 28, 2016

Page range: 177 - 180

Received: Oct 16, 2015

Accepted: May 16, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0026

Keywordscow, metritis, mast cell, histamine

© 2016 Guo-Qing Wang et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cow, metritis, mast cell, histamine