<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The current study was designed to detect <italic>Mycoplasma agalactiae</italic> (<italic>Ma</italic>), <italic>Mycoplasma mycoides</italic> subsp. <italic>capri</italic> (<italic>Mmc</italic>), <italic>Mycoplasma capricolum</italic> subsp. <italic>capricolum</italic> (<italic>Mcc</italic>) and <italic>Mycoplasma putrefaciens</italic> (<italic>Mp</italic>) in sheep and goats with clinical signs consistent with contagious agalactia.<bold>Material and Methods:</bold> A total of 299 samples were collected from 55 mixed herds in Azarbaijan-e-Sharghi province, Iran. Samples were examined using PCR and culture methods.<bold>Results:</bold> The findings showed that in 40 herds at least one sample was positive by PCR or culture method. Moreover, out of 274 sheep samples, 101 were proved to be positive using the PCR technique and 76 were found positive using the culture method. Out of 25 goat samples, 10 were found positive using PCR and 9 were positive through the culture method. Less than 20% of isolated mycoplasmas were <italic>Ma. Ma</italic> was detected from almost all studied regions in the province while <italic>Mmc</italic>, <italic>Mcc</italic>, and <italic>Mp</italic> were detected only in a very limited area that was deemed by the research group the mixed infection zone.<bold>Conclusion:</bold> In vaccination or eradication projects, it would be more economical to focus on mixed infection zones. Further investigation on mixed infection zones could facilitate better understanding of contagious agalactia epidemiology.</abstract>

Introduction: The current study was designed to detect Mycoplasma agalactiae (Ma), Mycoplasma mycoides subsp. capri (Mmc), Mycoplasma capricolum subsp. capricolum (Mcc) and Mycoplasma putrefaciens (Mp) in sheep and goats with clinical signs consistent with contagious agalactia.Material and Methods: A total of 299 samples were collected from 55 mixed herds in Azarbaijan-e-Sharghi province, Iran. Samples were examined using PCR and culture methods.Results: The findings showed that in 40 herds at least one sample was positive by PCR or culture method. Moreover, out of 274 sheep samples, 101 were proved to be positive using the PCR technique and 76 were found positive using the culture method. Out of 25 goat samples, 10 were found positive using PCR and 9 were positive through the culture method. Less than 20% of isolated mycoplasmas were Ma. Ma was detected from almost all studied regions in the province while Mmc, Mcc, and Mp were detected only in a very limited area that was deemed by the research group the mixed infection zone.Conclusion: In vaccination or eradication projects, it would be more economical to focus on mixed infection zones. Further investigation on mixed infection zones could facilitate better understanding of contagious agalactia epidemiology.

Mixed infection zones may be important in the epidemiology of contagious agalactia

Department of Microbiology, Faculty of Veterinary Sciences, Science and Research Branch, Islamic Azad University, 477893855, Tehran, Iran

Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, 3197619751, Karaj, Iran

Department of Aerobic Bacterial Vaccine Production, Razi Vaccine and Serum Research Institute, 3197619751, Karaj, Iran

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: The current study was designed to detect Mycoplasma agalactiae (Ma), Mycoplasma mycoides subsp. capri (Mmc), Mycoplasma capricolum subsp....

Mixed infection zones may be important in the epidemiology of contagious agalactia

Published Online: May 28, 2016

Page range: 159 - 162

Received: Nov 26, 2015

Accepted: May 12, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0023

Keywords
bovidae, contagious agalactia, bacteriological analysis, Iran

© 2016 Amirreza Jafarizadeh et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mixed infection zones may be important in the epidemiology of contagious agalactia

Published Online: May 28, 2016

Page range: 159 - 162

Received: Nov 26, 2015

Accepted: May 12, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0023

Keywordsbovidae, contagious agalactia, bacteriological analysis, Iran

© 2016 Amirreza Jafarizadeh et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
bovidae, contagious agalactia, bacteriological analysis, Iran