<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of the study was to analyze the involvement of the endothelium in the effects of neutrophils (PMNL) on phenylephrine-pre-contracted isolated rings of the rat thoracic aorta and to compare their effects with those of peroxynitrite (ONOO−) and hypochlorous acid (HOCl). Activated PMNL-induced contraction of the precontracted aorta was prevented by the blockade of NO-synthase and by endothelium removal. In the endothelium-free preparations, the effect of PMNL reappeared in the presence of sodium nitroprusside. The effect of ONOO− and HOCl significantly differed from that of activated PMNL both in the presence and absence of the endothelium. It is therefore likely that neither ONOO− nor HOCl generated by transformation of superoxide anion radical (O2•−) produced by PMNL is involved in their action. Reduction of the relaxant effect of nitric oxide derived from the endothelium by O2•− seems to be the keystone mechanism in generation of PMNL-induced contraction.</abstract>

The aim of the study was to analyze the involvement of the endothelium in the effects of neutrophils (PMNL) on phenylephrine-pre-contracted isolated rings of the rat thoracic aorta and to compare their effects with those of peroxynitrite (ONOO−) and hypochlorous acid (HOCl). Activated PMNL-induced contraction of the precontracted aorta was prevented by the blockade of NO-synthase and by endothelium removal. In the endothelium-free preparations, the effect of PMNL reappeared in the presence of sodium nitroprusside. The effect of ONOO− and HOCl significantly differed from that of activated PMNL both in the presence and absence of the endothelium. It is therefore likely that neither ONOO− nor HOCl generated by transformation of superoxide anion radical (O2•−) produced by PMNL is involved in their action. Reduction of the relaxant effect of nitric oxide derived from the endothelium by O2•− seems to be the keystone mechanism in generation of PMNL-induced contraction.

Endothelium and the effect of activated neutrophils on arterial smooth muscle

Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dúbravská cesta 9, 841 04 Bratislava, Slovak Republic

Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Endothelium and the effect of activated neutrophils on arterial smooth muscle"}

The aim of the study was to analyze the involvement of the endothelium in the effects of neutrophils (PMNL) on phenylephrine-pre-contracted isolated rings of...

Endothelium and the effect of activated neutrophils on arterial smooth muscle

Published Online: Dec 10, 2015

Page range: 44 - 47

Received: Feb 08, 2015

Accepted: Mar 12, 2015

DOI: https://doi.org/10.1515/intox-2015-0008

Keywords
rat thoracic aorta, endothelium, nitric oxide, activated neutrophils, hypochlorous acid, peroxynitrite

© 2015 Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Endothelium and the effect of activated neutrophils on arterial smooth muscle

Published Online: Dec 10, 2015

Page range: 44 - 47

Received: Feb 08, 2015

Accepted: Mar 12, 2015

DOI: https://doi.org/10.1515/intox-2015-0008

Keywordsrat thoracic aorta, endothelium, nitric oxide, activated neutrophils, hypochlorous acid, peroxynitrite

© 2015 Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
rat thoracic aorta, endothelium, nitric oxide, activated neutrophils, hypochlorous acid, peroxynitrite