<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Meloidogyne enterolobii (Me)</italic> is an emerging root-knot nematode (RKN) and the most important worldwide for its high aggressiveness and increasing geographical distribution. In Mexico only in watermelon and tomato has been reported. Here we report the presence of this nematode in Jalapeño pepper collected in the state of Sinaloa. The morphological and morphometric characteristics of the second-stage juveniles and adult females were similar to those described for <italic>Me</italic>. DNA sequencing and alignment analysis (BLAST/GenBank) showed that the SCAR and rDNA IGS2 fragments had a 99 % of identity, with <italic>Me</italic> DNA sequences present in the database. The results obtained indicate that the RKN detected corresponds to M. <italic>enterolobii</italic>. To our knowledge, this is the first report of this nematode in chilli pepper in this region of Mexico, considered of major economic importance for the variety of vegetables grown and the extension of the area sown.</p></abstract>

Meloidogyne enterolobii (Me) is an emerging root-knot nematode (RKN) and the most important worldwide for its high aggressiveness and increasing geographical distribution. In Mexico only in watermelon and tomato has been reported. Here we report the presence of this nematode in Jalapeño pepper collected in the state of Sinaloa. The morphological and morphometric characteristics of the second-stage juveniles and adult females were similar to those described for Me. DNA sequencing and alignment analysis (BLAST/GenBank) showed that the SCAR and rDNA IGS2 fragments had a 99 % of identity, with Me DNA sequences present in the database. The results obtained indicate that the RKN detected corresponds to M. enterolobii. To our knowledge, this is the first report of this nematode in chilli pepper in this region of Mexico, considered of major economic importance for the variety of vegetables grown and the extension of the area sown.

Presence of <italic>Meloidogyne enterolobii</italic> on Jalapeño pepper (<italic>Capsicum annuum</italic> L.) in Sinaloa, Mexico

Programa de Fitopatología, Colegio de Postgraduados-Campus Montecillo, km 36.5 Carretera México-Texcoco, Texcoco, Estado de México, 56230, México

: Instituto Politécnico Nacional, CIIDIR-IPN Unidad Michoacán. Justo Sierra 28, 59510, Jiquilpan, Michoacán, México

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Presence of  on Jalapeño pepper ( L.) in Sinaloa, Mexico

Meloidogyne enterolobii (Me) is an emerging root-knot nematode (RKN) and the most important worldwide for its high aggressiveness and increasing geographical...

Character	This work	Yang and Eisenback, 1983	Rammah and Hirschmann, 1988

		Second-stage juveniles (J₂)
	n=28	n=30	n=35
Body length	414.5 ±22.9# (391.5–486.8) ^#Average measurements in μm ± standard deviation (range) a: body length/greatest body width, c: body length/tail length DGO: dorsal esophageal gland orifice	436.6 ±16.61 (405–472.9)	453.6 ± 28.4 (390.4–528.0)
Greatest body width	14.05 ±0.7 (13.2–15.6)	15.3 ±0.89 (13.9–17.8)	14.7 ±0.5 (13.8–15.8)
Stylet length	11.3 ±0.8 (10.1–13.0)	11.7 ±0.45 (10.8–13.0)	11.6 ±0.3 (11.1–12.2)
DGO	3.4 ±0.4 (2.6–4.2)	3.4 ± 0.33 (2.8–4.3)	3.9 ±0.2 (3.3–4.3)
Tail length	54.4 ± 5.5 (40.7–61.6)	56.4 ± 4.48 (41.5–63.4)	54.4 ± 3.6 (49.2 - 62.9)
Hyaline region length	14.2 ±0.8 (11.5–15.0)	------------	-------------
a	29.5 ±1.6 (26.3–33.5)	28.6 ±1.88 (24.0 - 32.5)	30.9 ±1.9 (26.4–34.7)
c	7.9 ±0.7 (6.4–9.6)	7.8 ±0.65 (6.8–10.1)	8.3 ±0.4 (7.0–9.2)

		Females
	n=8	n=20	n=35

Stylet length	14.5 ±1.7 (12.8–17.3)	15.1 ±1.35 (13.2–18.0)	15.8 ±0.8 (13.8–16.8)
DGO	4.7 ±1.1 (3.2 - 6.4)	4.9 ±0.78 (3.7–6.2)	4.8 ±0.8 (3.5–6.7)
Vulva-anus distance	18.5 ±1.0 (17.4–19.6)	22.2 ±1.76 (19.7–26.6)	18.4 ±1.5 (12.7–21.1)
Vulval slit length	23.5 ±1.9 (20.2 - 25.3)	28.7 ± 1.96 (25.3–32.4)	26.1 ± 1.9 (20.9–30.4)

Presence of Meloidogyne enterolobii on Jalapeño pepper (Capsicum annuum L.) in Sinaloa, Mexico

Article Category: Research Article

Published Online: Mar 26, 2016

Page range: 155 - 160

Received: Jun 23, 2015

Accepted: Sep 17, 2015

DOI: https://doi.org/10.1515/helmin-2016-0001

Keywords
PCR; sequence-characterized amplified region, intergenic spacer region, root-knot nematodes

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Morphometry of second-stage juveniles and adult females from chilli pepper (cv. Jalapeño) compared with previous data of M. enterolobii described n the literature.

Presence of Meloidogyne enterolobii on Jalapeño pepper (Capsicum annuum L.) in Sinaloa, Mexico

Article Category: Research Article

Published Online: Mar 26, 2016

Page range: 155 - 160

Received: Jun 23, 2015

Accepted: Sep 17, 2015

DOI: https://doi.org/10.1515/helmin-2016-0001

KeywordsPCR; sequence-characterized amplified region, intergenic spacer region, root-knot nematodes

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Morphometry of second-stage juveniles and adult females from chilli pepper (cv. Jalapeño) compared with previous data of M. enterolobii described n the literature.

Keywords
PCR; sequence-characterized amplified region, intergenic spacer region, root-knot nematodes