<abstract xmlns="http://www.w3.org/1999/xhtml"> This study investigated the potential genotoxic effects of the fungicide Tango® Super using methods of conventional cytogenetic analysis, fluorescence in situ hybridization (FISH) and detection of DNA fragmentation in bovine lymphocytes. After exposure of two donor cell cultures to several concentrations of fungicide (0.5, 3.0 and 15.0 mg.ml-1 for conventional cytogenetic analysis; 0.5 and 3.0 mg.ml-1 for FISH) we detected the insignificant occurrence of chromosome and chromatid breakages. In both donors we observed a significant decrease in mitotic index (MI) percentage with increasing concentrations of fungicide (P &lt; 0.01; P &lt; 0.001), which indicated a cytotoxic effect of the preparation. Electrophoretic analysis of DNA fragmentation in lymphocytes exposed to increasing concentrations (0.5; 1.5; 3.0; 6.0 and 15.0 mg.ml-1) of this preparation showed its ability to induce formation of fragments, which is a characteristic manifestation of the last stage of apoptosis.</abstract>

This study investigated the potential genotoxic effects of the fungicide Tango® Super using methods of conventional cytogenetic analysis, fluorescence in situ hybridization (FISH) and detection of DNA fragmentation in bovine lymphocytes. After exposure of two donor cell cultures to several concentrations of fungicide (0.5, 3.0 and 15.0 mg.ml-1 for conventional cytogenetic analysis; 0.5 and 3.0 mg.ml-1 for FISH) we detected the insignificant occurrence of chromosome and chromatid breakages. In both donors we observed a significant decrease in mitotic index (MI) percentage with increasing concentrations of fungicide (P < 0.01; P < 0.001), which indicated a cytotoxic effect of the preparation. Electrophoretic analysis of DNA fragmentation in lymphocytes exposed to increasing concentrations (0.5; 1.5; 3.0; 6.0 and 15.0 mg.ml-1) of this preparation showed its ability to induce formation of fragments, which is a characteristic manifestation of the last stage of apoptosis.

Evaluation of the Genotoxic Effect of the Commercial Fungicide Tango® Super on Bovine Lymphocytes

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Evaluation of the Genotoxic Effect of the Commercial Fungicide Tango Super on Bovine Lymphocytes

This study investigated the potential genotoxic effects of the fungicide Tango® Super using methods of conventional cytogenetic analysis, fluorescence in situ...

Evaluation of the Genotoxic Effect of the Commercial Fungicide Tango^® Super on Bovine Lymphocytes

Published Online: Dec 29, 2017

Page range: 28 - 33

Received: Jul 01, 2017

Accepted: Sep 27, 2017

DOI: https://doi.org/10.1515/fv-2017-0035

Keywords
chromosomal analysis, DNA fragmentation, fluorescence in situ hybridization, lymphocyte culture, systemic fungicide

© 2018

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Evaluation of the Genotoxic Effect of the Commercial Fungicide Tango® Super on Bovine Lymphocytes

Published Online: Dec 29, 2017

Page range: 28 - 33

Received: Jul 01, 2017

Accepted: Sep 27, 2017

DOI: https://doi.org/10.1515/fv-2017-0035

Keywordschromosomal analysis, DNA fragmentation, fluorescence in situ hybridization, lymphocyte culture, systemic fungicide

© 2018

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Evaluation of the Genotoxic Effect of the Commercial Fungicide Tango^® Super on Bovine Lymphocytes

Keywords
chromosomal analysis, DNA fragmentation, fluorescence in situ hybridization, lymphocyte culture, systemic fungicide