<abstract xmlns="http://www.w3.org/1999/xhtml">Undesirable substances enter the organism of animals mostly via feed, water or veterinary medicines and their residues pass subsequently into the products of animal origin. In dairy cows, sheep and goats these residues are eliminated particularly in milk. Milk intended for human consumption must comply with safety criteria also with respect to residues of antibiotics. The aim of this study was to determine the presence or absence of antibiotic residues in the milk using the tests Milchtest and Premi®Test. While the Milchtest was developed for the determination of antibiotic residues in cow, sheep and goat milk, the Premi®Test is intended for the determination of antibiotic residues in meat juice, liver, kidneys, fish, eggs and in the urine of animals treated with antibiotics. As examined matrices, we used 45 samples of raw cow’s milk collected at 3 agricultural farms and 10 samples of milk offered to consumers at grocery stores. When using the Milchtest, 8 samples tested positive and 10 provided dubious results while testing with the Premi®Test showed that only 6 samples were positive for antibiotics. Comparison of the results confirmed a higher detection sensitivity of Milchtest reflected in higher numbers of positive samples and the detection of dubious results in samples of raw cow’s milk. However, it should be noted that even the Premi®Test, although not intended preferably for the determination of antibiotics in milk, can be used, if needed, for the preliminary screening of antibiotic residues in such a matrix.</abstract>

Undesirable substances enter the organism of animals mostly via feed, water or veterinary medicines and their residues pass subsequently into the products of animal origin. In dairy cows, sheep and goats these residues are eliminated particularly in milk. Milk intended for human consumption must comply with safety criteria also with respect to residues of antibiotics. The aim of this study was to determine the presence or absence of antibiotic residues in the milk using the tests Milchtest and Premi®Test. While the Milchtest was developed for the determination of antibiotic residues in cow, sheep and goat milk, the Premi®Test is intended for the determination of antibiotic residues in meat juice, liver, kidneys, fish, eggs and in the urine of animals treated with antibiotics. As examined matrices, we used 45 samples of raw cow’s milk collected at 3 agricultural farms and 10 samples of milk offered to consumers at grocery stores. When using the Milchtest, 8 samples tested positive and 10 provided dubious results while testing with the Premi®Test showed that only 6 samples were positive for antibiotics. Comparison of the results confirmed a higher detection sensitivity of Milchtest reflected in higher numbers of positive samples and the detection of dubious results in samples of raw cow’s milk. However, it should be noted that even the Premi®Test, although not intended preferably for the determination of antibiotics in milk, can be used, if needed, for the preliminary screening of antibiotic residues in such a matrix.

Determination of Antibiotic Residues in Milk by Microbial Inhibitory Tests

University of Veterinary Medicine and Pharmacy

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Determination of Antibiotic Residues in Milk by Microbial Inhibitory Tests"}

Undesirable substances enter the organism of animals mostly via feed, water or veterinary medicines and their residues pass subsequently into the products of...

Determination of Antibiotic Residues in Milk by Microbial Inhibitory Tests

Published Online: Oct 11, 2017

Page range: 57 - 64

Received: May 22, 2017

Accepted: Aug 19, 2017

DOI: https://doi.org/10.1515/fv-2017-0028

Keywords
antibiotics, milk, residues, screening

© 2017 Juščáková, D. et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Determination of Antibiotic Residues in Milk by Microbial Inhibitory Tests

Published Online: Oct 11, 2017

Page range: 57 - 64

Received: May 22, 2017

Accepted: Aug 19, 2017

DOI: https://doi.org/10.1515/fv-2017-0028

Keywordsantibiotics, milk, residues, screening

© 2017 Juščáková, D. et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
antibiotics, milk, residues, screening