<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of this study was to develop methods for the <italic>in vitro</italic> propagation of <italic>Zantedeschia rehmannii</italic>. <italic>Zantedeschia rehmannii</italic> tuber fragments (1 cm2) containing eyes were soaked for 30 s in a solution containing 100 mg dm−3 L-ascorbic acid (AA) before transfer to culture vessels containing an MS medium supplemented with BAP (0 to 3 mg dm−3). Cultures were maintained in darkness. Soaking explants in an L-ascorbic acid solution improved the establishment of explants. Culture initiation should be conducted on media supplemented with 3 mg dm−3 BAP. On a multiplication stage, adventitious shoots were placed on MS media supplemented with cytokinin: BAP (0.5 to 5 mg dm−3), KIN (0.5 to 5 mg dm−3), TDZ (0.1 to 1 mg dm−3) and 2iP (2.5 to 15 mg dm−3) or BAP (0.5 to 7.5 mg dm−3) with IAA (0.5 to 2 mg dm−3). The highest coefficient of multiplication for <italic>Zantedeschia</italic> was obtained on the medium with the addition of 2.5 mg dm−3 BAP, which positively affected the shoot length (3.41 cm) and the number of adventitious shoots (4.13). Rooting took place on media supplemented with IBA, IAA and NAA at a concentration of 0.1 to 2 mg dm−3. The most numerous and the longest roots were found in plants placed on a medium with the addition of 0.1 mg dm−3 IBA.</abstract>

The aim of this study was to develop methods for the in vitro propagation of Zantedeschia rehmannii. Zantedeschia rehmannii tuber fragments (1 cm2) containing eyes were soaked for 30 s in a solution containing 100 mg dm−3 L-ascorbic acid (AA) before transfer to culture vessels containing an MS medium supplemented with BAP (0 to 3 mg dm−3). Cultures were maintained in darkness. Soaking explants in an L-ascorbic acid solution improved the establishment of explants. Culture initiation should be conducted on media supplemented with 3 mg dm−3 BAP. On a multiplication stage, adventitious shoots were placed on MS media supplemented with cytokinin: BAP (0.5 to 5 mg dm−3), KIN (0.5 to 5 mg dm−3), TDZ (0.1 to 1 mg dm−3) and 2iP (2.5 to 15 mg dm−3) or BAP (0.5 to 7.5 mg dm−3) with IAA (0.5 to 2 mg dm−3). The highest coefficient of multiplication for Zantedeschia was obtained on the medium with the addition of 2.5 mg dm−3 BAP, which positively affected the shoot length (3.41 cm) and the number of adventitious shoots (4.13). Rooting took place on media supplemented with IBA, IAA and NAA at a concentration of 0.1 to 2 mg dm−3. The most numerous and the longest roots were found in plants placed on a medium with the addition of 0.1 mg dm−3 IBA.

Micropropagation of calla lily (<italic>Zantedeschia rehmannii</italic>)

Department of Plant Genetic, Breeding and Biotechnology, Faculty of Environmental Management and Agriculture, West Pomeranian University of Technology, Slowackiego 17, 71-434 Szczecin,

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The aim of this study was to develop methods for the in vitro propagation of Zantedeschia rehmannii. Zantedeschia rehmannii tuber fragments (1 cm2) containing...

Micropropagation of calla lily (Zantedeschia rehmannii)

Published Online: Dec 28, 2016

Page range: 181 - 186

Received: Apr 28, 2016

Accepted: Nov 09, 2016

DOI: https://doi.org/10.1515/fhort-2016-0021

Keywords
antioxidant, Araceae, L-ascorbic acid, propagation

© 2016 Danuta Kulpa, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Micropropagation of calla lily (Zantedeschia rehmannii)

Published Online: Dec 28, 2016

Page range: 181 - 186

Received: Apr 28, 2016

Accepted: Nov 09, 2016

DOI: https://doi.org/10.1515/fhort-2016-0021

Keywordsantioxidant, Araceae, L-ascorbic acid, propagation

© 2016 Danuta Kulpa, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
antioxidant, Araceae, L-ascorbic acid, propagation