<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>An analytical validation of a screening ELISA for detection of chloramphenicol (CAP) in honey was conducted according to the Commission Decision 2002/657/EC and Guidelines for the Validation of Screening Methods for Residues of Veterinary Medicines. The analyte was extracted from honey with a water and ethyl acetate mixture, and CAP concentrations were measured photometrically at 450 nm. The recovery rate of the analyte from spiked samples was 79%. The cut-off level of CAP in honey as the minimum recovery (0.17 units) was established. Detection capability (CCβ) was fixed at 0.25 μg kg<sup>−1</sup>. No relevant interferences between matrix effects and structurally related substances including florfenicol and thiamphenicol were observed. The ELISA method should be useful for determination of CAP residues in honey monitoring.</p>
</abstract>

An analytical validation of a screening ELISA for detection of chloramphenicol (CAP) in honey was conducted according to the Commission Decision 2002/657/EC and Guidelines for the Validation of Screening Methods for Residues of Veterinary Medicines. The analyte was extracted from honey with a water and ethyl acetate mixture, and CAP concentrations were measured photometrically at 450 nm. The recovery rate of the analyte from spiked samples was 79%. The cut-off level of CAP in honey as the minimum recovery (0.17 units) was established. Detection capability (CCβ) was fixed at 0.25 μg kg−1. No relevant interferences between matrix effects and structurally related substances including florfenicol and thiamphenicol were observed. The ELISA method should be useful for determination of CAP residues in honey monitoring.

ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Department of Pharmacology and Toxicology

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

An analytical validation of a screening ELISA for detection of chloramphenicol (CAP) in honey was conducted according to the Commission Decision 2002/657/EC...

ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Published Online: Sep 30, 2015

Page range: 353 - 356

Received: Mar 13, 2015

Accepted: Sep 03, 2015

DOI: https://doi.org/10.1515/bvip-2015-0052

Keywords
honey, chloramphenicol, ELISA, validation

© 2015 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Published Online: Sep 30, 2015

Page range: 353 - 356

Received: Mar 13, 2015

Accepted: Sep 03, 2015

DOI: https://doi.org/10.1515/bvip-2015-0052

Keywordshoney, chloramphenicol, ELISA, validation

© 2015 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
honey, chloramphenicol, ELISA, validation