<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Chemical and genetic characterization of <italic>Veratrum</italic> species deposited in European collections is important for genepool preservation and identification of populations with desired metabolic properties. <italic>Veratrum album, V. lobelianum</italic> and <italic>V. nigrum</italic> are native to Europe, and in Poland are ranked as rare or threatened. Genetic variation of European <italic>Veratrum</italic> species was characterized by Amplified Fragment Length Polymorphism (AFLP) markers. The accumulation of jervine as a representative of steroidal alkaloids was measured in seeds. Distribution of 380 markers generated from eight primer combinations was useful for studying genetic relationships among and within species in the <italic>Veratrum</italic> genus and the most divergent populations were identified. Genetic variation between 12 populations of <italic>Veratrum</italic> species supports the classification of <italic>V. lobelianum</italic> as a subspecies of <italic>V. album</italic>. However, the results need further validation on extended material. A higher genetic diversity (22.3%) was observed between populations of <italic>V. nigrum</italic> as compared to <italic>V. album</italic> (14.5%). Contents of jervine allowed for discrimination of the studied <italic>Veratrum</italic> species and can be used as a potential chemotaxonomic marker. The highest jervine levels were found in <italic>V. album. V. nigrum</italic> seeds had only trace amounts and no jervine was detected in seeds of <italic>V. lobelianum</italic>.</p></abstract>

Chemical and genetic characterization of Veratrum species deposited in European collections is important for genepool preservation and identification of populations with desired metabolic properties. Veratrum album, V. lobelianum and V. nigrum are native to Europe, and in Poland are ranked as rare or threatened. Genetic variation of European Veratrum species was characterized by Amplified Fragment Length Polymorphism (AFLP) markers. The accumulation of jervine as a representative of steroidal alkaloids was measured in seeds. Distribution of 380 markers generated from eight primer combinations was useful for studying genetic relationships among and within species in the Veratrum genus and the most divergent populations were identified. Genetic variation between 12 populations of Veratrum species supports the classification of V. lobelianum as a subspecies of V. album. However, the results need further validation on extended material. A higher genetic diversity (22.3%) was observed between populations of V. nigrum as compared to V. album (14.5%). Contents of jervine allowed for discrimination of the studied Veratrum species and can be used as a potential chemotaxonomic marker. The highest jervine levels were found in V. album. V. nigrum seeds had only trace amounts and no jervine was detected in seeds of V. lobelianum.

Genetic diversity of three European <italic>Veratrum</italic> species revealed by Amplified Fragment Length Polymorphism

Department of Biotechnology and Bioinformatics, Faculty of Chemistry

Biodiversity Research and Conservation

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic diversity of three European  species revealed by Amplified Fragment Length Polymorphism

Chemical and genetic characterization of Veratrum species deposited in European collections is important for genepool preservation and identification of...

Genetic diversity of three European Veratrum species revealed by Amplified Fragment Length Polymorphism

Published Online: Oct 16, 2018

Page range: 1 - 8

Received: Jun 13, 2017

Accepted: Sep 30, 2017

DOI: https://doi.org/10.1515/biorc-2017-0011

Keywords
genetic variation, hellebore, jervine, Melanthiaceae, molecular markers

© 2017 Magdalena Szeliga et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic diversity of three European Veratrum species revealed by Amplified Fragment Length Polymorphism

Published Online: Oct 16, 2018

Page range: 1 - 8

Received: Jun 13, 2017

Accepted: Sep 30, 2017

DOI: https://doi.org/10.1515/biorc-2017-0011

Keywordsgenetic variation, hellebore, jervine, Melanthiaceae, molecular markers

© 2017 Magdalena Szeliga et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
genetic variation, hellebore, jervine, Melanthiaceae, molecular markers