<abstract xmlns="http://www.w3.org/1999/xhtml">The objective of the present study was to obtain the optimum proportion of the carbon source and prebiotics for <italic>Bifidobacterium bifidum BB01</italic> by the central composite design (CCD). The effect of carbon source (lactose) and two prebiotics (inulin and fructooligosaccharides) on the BB01 were observed by measuring the OD600 value, pH value and the viable counts at 18h. The final optimized concentrations of carbon source and prebiotics were: lactose 1.6%, inulin 0.26%, and fructooligosaccharides 0.22%. The result indicates that the growth of <italic>B. bifidum BB01</italic> shows an significant increase in the optimized culture medium (p &lt; 0.05), the OD600 value reached 1.434 at 18h, which increased 6.58% compared to the control. And the viable counts of <italic>B. bifidum BB01</italic> increased 24.36% and reached (2.17±0.06) ×109cfu/mL. The results show that the optimization of the carbon source and prebiotics using CCD in this study is workable and necessary.</abstract>

The objective of the present study was to obtain the optimum proportion of the carbon source and prebiotics for Bifidobacterium bifidum BB01 by the central composite design (CCD). The effect of carbon source (lactose) and two prebiotics (inulin and fructooligosaccharides) on the BB01 were observed by measuring the OD600 value, pH value and the viable counts at 18h. The final optimized concentrations of carbon source and prebiotics were: lactose 1.6%, inulin 0.26%, and fructooligosaccharides 0.22%. The result indicates that the growth of B. bifidum BB01 shows an significant increase in the optimized culture medium (p < 0.05), the OD600 value reached 1.434 at 18h, which increased 6.58% compared to the control. And the viable counts of B. bifidum BB01 increased 24.36% and reached (2.17±0.06) ×109cfu/mL. The results show that the optimization of the carbon source and prebiotics using CCD in this study is workable and necessary.

Application of central composite design to optimize the amount of carbon source and prebiotics for Bifidobacterium bifidum BB01

Shaanxi University of Science & Technology

Acta Universitatis Cibiniensis. Series E: Food Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The objective of the present study was to obtain the optimum proportion of the carbon source and prebiotics for Bifidobacterium bifidum BB01 by the central...

Application of central composite design to optimize the amount of carbon source and prebiotics for Bifidobacterium bifidum BB01

Published Online: Jun 18, 2016

Page range: 41 - 52

DOI: https://doi.org/10.1515/aucft-2016-0003

Keywords
central composite design, prebiotics, probiotics

© 2016 Lucian Blaga University, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Application of central composite design to optimize the amount of carbon source and prebiotics for Bifidobacterium bifidum BB01

Published Online: Jun 18, 2016

Page range: 41 - 52

DOI: https://doi.org/10.1515/aucft-2016-0003

Keywordscentral composite design, prebiotics, probiotics

© 2016 Lucian Blaga University, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
central composite design, prebiotics, probiotics