<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of this work is to describe a covalent immobilization of antibodies onto the poly- acrylamide-acrylonitrile or hybrid material UREASIL and creation of optical immunosensor for determination of aflatoxin Bl. For this purpose, mouse-anti-aflatoxin B1 antibodies with oxidized carbohydrate moieties were covalently immobilized on the membranes of polyacrylamide- polyacrylonitrile copolymer, as well as the hybrid material UREASIL. To determine the affinity> binding of the immobilized antibody with afatoxin Bl was used "sandwich" method. Associated with the immobilized antibody sought ingredients interact with a surplus of secondary’ signal antibodies. The described method has been developed as a model system, which can easily be applied for the determination of aflatoxins in samples of different origin. To the best of our knowledge, this is the first study to show that in the establishment of biosensor was used hybrid material UREASIL.</p></abstract>

The aim of this work is to describe a covalent immobilization of antibodies onto the poly- acrylamide-acrylonitrile or hybrid material UREASIL and creation of optical immunosensor for determination of aflatoxin Bl. For this purpose, mouse-anti-aflatoxin B1 antibodies with oxidized carbohydrate moieties were covalently immobilized on the membranes of polyacrylamide- polyacrylonitrile copolymer, as well as the hybrid material UREASIL. To determine the affinity> binding of the immobilized antibody with afatoxin Bl was used "sandwich" method. Associated with the immobilized antibody sought ingredients interact with a surplus of secondary’ signal antibodies. The described method has been developed as a model system, which can easily be applied for the determination of aflatoxins in samples of different origin. To the best of our knowledge, this is the first study to show that in the establishment of biosensor was used hybrid material UREASIL.

Covalent immobilization of rabbit-antiaflatoxin-antibodies onto the poly-acrylamideacrylonitrile as well as hybrid material UREASIL and developing an optical immunosensor

Institute of Electrochemistry and Energy Sources, Bulgarian Academy of Science, Sofia, Bulgaria

Department of Machine Elements and Chemistry, Faculty of Transport Management, Todor Kableshkov University of Transport, Sofia, Bulgaria

Department of Inorganic Chemistry and Chemical Education, Faculty of Natural Sciences, Shumen University “Konstantin Preslavski”, Shumen,

Middlesex University, School of Health and Education-London, United Kingdom

United Kingdom of Great Britain and Northern Ireland

Acta Scientifica Naturalis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The aim of this work is to describe a covalent immobilization of antibodies onto the poly- acrylamide-acrylonitrile or hybrid material UREASIL and creation of...

Covalent immobilization of rabbit-antiaflatoxin-antibodies onto the poly-acrylamideacrylonitrile as well as hybrid material UREASIL and developing an optical immunosensor

Published Online: Feb 09, 2017

Page range: 52 - 56

Received: Oct 30, 2016

Accepted: Jan 11, 2017

DOI: https://doi.org/10.1515/asn-2017-0008

Keywords
aflatoxin B1, immunosensor, optical Biosensor, immobilization, antibody

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Covalent immobilization of rabbit-antiaflatoxin-antibodies onto the poly-acrylamideacrylonitrile as well as hybrid material UREASIL and developing an optical immunosensor

Published Online: Feb 09, 2017

Page range: 52 - 56

Received: Oct 30, 2016

Accepted: Jan 11, 2017

DOI: https://doi.org/10.1515/asn-2017-0008

Keywordsaflatoxin B1, immunosensor, optical Biosensor, immobilization, antibody

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
aflatoxin B1, immunosensor, optical Biosensor, immobilization, antibody