<abstract xmlns="http://www.w3.org/1999/xhtml">
				
				<p> The purpose of the study was to verify the hypothesis that there are doses of silver nanoparticles (Ag-NPs) that improve immune defence in chickens without compromising their health. To verify this hypothesis, an attempt was made to determine which doses of Ag-NPs (5 nm) consumed by chickens as a result of administration of hydrocolloids during varying time periods at a concentration of 5 or 10 mg Ag/l stimulate immune defence. The experiment was performed on 296 chickens assigned to 3 treatments. Chickens from the control treatment received drinking water without experimental additives. Chickens from the T-5 and T-10 treatments received a hydrocolloid of Ag-NPs at a concentration of 5 mg/l (treatment T-5) or 10 mg/l (treatment T-10) from their second week of life. Blood for analysis was collected at the age of 42 days from 8 birds per treatment. Ceruloplasmin (Cp), leukocyte count (WBC), erythrocyte sedimentation rate (ESR), interleukin IL-6, immunoglobulins IgA, IgY, phagocytic cells (% PC), phagocytic index (PI), nitroblue tetrazolium reduction (NBT), and lysozyme content in the blood was determined. Application of Ag-NPs at a concentration of 5 mg/l or 10 mg/l in the dose range of 2.87-12.25 mg/bird (administration of Ag-NPs in concentration 5 mg/l in weeks: 2; 2 and 3; 2 and 4; 2 and 5; 2 and 6 or concentration 10 mg/l in week 2) resulted in an immunostimulatory effect expressed as an increase in heterophil respiratory burst and an increased concentration of lysozyme. Higher doses of Ag-NPs exerted a pro-inflammatory effect, as indicated by elevated levels of IL-6 and ceruloplasmin, as well as a high ESR. They also stimulated B lymphocytes to produce IgA and IgA immunoglobulins.</p>
			</abstract>

The purpose of the study was to verify the hypothesis that there are doses of silver nanoparticles (Ag-NPs) that improve immune defence in chickens without compromising their health. To verify this hypothesis, an attempt was made to determine which doses of Ag-NPs (5 nm) consumed by chickens as a result of administration of hydrocolloids during varying time periods at a concentration of 5 or 10 mg Ag/l stimulate immune defence. The experiment was performed on 296 chickens assigned to 3 treatments. Chickens from the control treatment received drinking water without experimental additives. Chickens from the T-5 and T-10 treatments received a hydrocolloid of Ag-NPs at a concentration of 5 mg/l (treatment T-5) or 10 mg/l (treatment T-10) from their second week of life. Blood for analysis was collected at the age of 42 days from 8 birds per treatment. Ceruloplasmin (Cp), leukocyte count (WBC), erythrocyte sedimentation rate (ESR), interleukin IL-6, immunoglobulins IgA, IgY, phagocytic cells (% PC), phagocytic index (PI), nitroblue tetrazolium reduction (NBT), and lysozyme content in the blood was determined. Application of Ag-NPs at a concentration of 5 mg/l or 10 mg/l in the dose range of 2.87-12.25 mg/bird (administration of Ag-NPs in concentration 5 mg/l in weeks: 2; 2 and 3; 2 and 4; 2 and 5; 2 and 6 or concentration 10 mg/l in week 2) resulted in an immunostimulatory effect expressed as an increase in heterophil respiratory burst and an increased concentration of lysozyme. Higher doses of Ag-NPs exerted a pro-inflammatory effect, as indicated by elevated levels of IL-6 and ceruloplasmin, as well as a high ESR. They also stimulated B lymphocytes to produce IgA and IgA immunoglobulins.

The Effect of Administration of Silver Nanoparticles on the Immune Status of Chickens

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The purpose of the study was to verify the hypothesis that there are doses of silver nanoparticles (Ag-NPs) that improve immune defence in chickens without...

The Effect of Administration of Silver Nanoparticles on the Immune Status of Chickens

Article Category: The Biology, Physiology, Reproduction, and Health

Published Online: May 11, 2018

Page range: 401 - 416

Received: Sep 13, 2017

Accepted: Nov 29, 2017

DOI: https://doi.org/10.1515/aoas-2017-0043

Keywords
silver nanoparticles, chicken, blood, immune parameters, haematological indices

© by Katarzyna Ognik

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The Effect of Administration of Silver Nanoparticles on the Immune Status of Chickens

Article Category: The Biology, Physiology, Reproduction, and Health

Published Online: May 11, 2018

Page range: 401 - 416

Received: Sep 13, 2017

Accepted: Nov 29, 2017

DOI: https://doi.org/10.1515/aoas-2017-0043

Keywordssilver nanoparticles, chicken, blood, immune parameters, haematological indices

© by Katarzyna Ognik

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
silver nanoparticles, chicken, blood, immune parameters, haematological indices