<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Quercetin is a natural flavonoid with strong antioxidant activity. In the present study, we evaluate the influence of different concentrations of quercetin (QT) on intracytoplasmic oxidative stress and glutathione (GSH) concentration, during in vitro maturation (IVM) and fertilization in mouse oocytes. IVM was carried out in the presence of control (QT0), 5 (QT5), 10 (QT10), and 20 (QT20) μg/mL of QT. Nuclear maturation, intracellular GSH and ROS content were evaluated following the IVM. In these oocytes, we subsequently evaluated the effect of QT supplementation on embryo development, including 2-cell, 8-cell, and blastocyst rate. The results of the present study showed that the supplementation of 10 μg/mL QT in maturation medium increased the number of MII oocytes. In addition, fertilization and blastocyst rate in QT10 treatment group were significantly higher in comparison to the other groups, and elevated the amount of intracellular GSH content compared to other QT concentrations and control groups. The intracellular ROS level was the lowest among oocytes matured in Q5 and Q10 treatment groups. This result suggested that quercetin dose-dependently improves nuclear maturation and embryo development, via reducing intracytoplasmic oxidative stress in mature oocyte.</p></abstract>

Quercetin is a natural flavonoid with strong antioxidant activity. In the present study, we evaluate the influence of different concentrations of quercetin (QT) on intracytoplasmic oxidative stress and glutathione (GSH) concentration, during in vitro maturation (IVM) and fertilization in mouse oocytes. IVM was carried out in the presence of control (QT0), 5 (QT5), 10 (QT10), and 20 (QT20) μg/mL of QT. Nuclear maturation, intracellular GSH and ROS content were evaluated following the IVM. In these oocytes, we subsequently evaluated the effect of QT supplementation on embryo development, including 2-cell, 8-cell, and blastocyst rate. The results of the present study showed that the supplementation of 10 μg/mL QT in maturation medium increased the number of MII oocytes. In addition, fertilization and blastocyst rate in QT10 treatment group were significantly higher in comparison to the other groups, and elevated the amount of intracellular GSH content compared to other QT concentrations and control groups. The intracellular ROS level was the lowest among oocytes matured in Q5 and Q10 treatment groups. This result suggested that quercetin dose-dependently improves nuclear maturation and embryo development, via reducing intracytoplasmic oxidative stress in mature oocyte.

Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Quercetin is a natural flavonoid with strong antioxidant activity. In the present study, we evaluate the influence of different concentrations of quercetin...

Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Published Online: Jan 30, 2018

Page range: 87 - 98

Received: Mar 27, 2017

Accepted: Sep 12, 2017

DOI: https://doi.org/10.1515/aoas-2017-0029

Keywords
in vitro maturation, quercetin, oxidative stress, glutathione

© by Marefat Ghaffari Novin

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Published Online: Jan 30, 2018

Page range: 87 - 98

Received: Mar 27, 2017

Accepted: Sep 12, 2017

DOI: https://doi.org/10.1515/aoas-2017-0029

Keywordsin vitro maturation, quercetin, oxidative stress, glutathione

© by Marefat Ghaffari Novin

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
in vitro maturation, quercetin, oxidative stress, glutathione