<abstract xmlns="http://www.w3.org/1999/xhtml"><p>European Union regulations (e.g. Commission Regulation (EU) No 56/2013) set restrictions on the use of poultry meals in animal nutrition, which requires the species composition of manufactured feeds to be constantly monitored. The aim of this study is to develop a method for qualitative analysis of poultry meals, enabling the four main poultry species (chicken, turkey, duck, goose) to be detected using one pair of primers, and a method for quantitative determination of chicken meals in poultry meals. The qualitative identification method was developed using PCR technology, whereas qPCR and TaqMan probes were used for quantitative identification. The study was performed with samples of feed mixture containing poultry and chicken meal. The limit of determination was 0.08% and 0.02% for qualitative and quantitative identification, respectively. The results of quantitative determinations obtained for independent DNA isolations from the same samples are repeatable (RSDcT ≤0.46%). The determined concentrations are accurate (Dc ≤11.23% for c ≥0.06). The identification of target sequences in both tests is good enough for commercial applications.</p></abstract>

European Union regulations (e.g. Commission Regulation (EU) No 56/2013) set restrictions on the use of poultry meals in animal nutrition, which requires the species composition of manufactured feeds to be constantly monitored. The aim of this study is to develop a method for qualitative analysis of poultry meals, enabling the four main poultry species (chicken, turkey, duck, goose) to be detected using one pair of primers, and a method for quantitative determination of chicken meals in poultry meals. The qualitative identification method was developed using PCR technology, whereas qPCR and TaqMan probes were used for quantitative identification. The study was performed with samples of feed mixture containing poultry and chicken meal. The limit of determination was 0.08% and 0.02% for qualitative and quantitative identification, respectively. The results of quantitative determinations obtained for independent DNA isolations from the same samples are repeatable (RSDcT ≤0.46%). The determined concentrations are accurate (Dc ≤11.23% for c ≥0.06). The identification of target sequences in both tests is good enough for commercial applications.

The use of PCR and Real-Time PCR for Qualitative and Quantitative Determination of Poultry and Chicken Meals

Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

European Union regulations (e.g. Commission Regulation (EU) No 56/2013) set restrictions on the use of poultry meals in animal nutrition, which requires the...

The use of PCR and Real-Time PCR for Qualitative and Quantitative Determination of Poultry and Chicken Meals

Published Online: Aug 02, 2016

Page range: 731 - 741

Received: Jun 22, 2015

Accepted: Jan 29, 2016

DOI: https://doi.org/10.1515/aoas-2016-0003

Keywords
poultry meal, species identification, PCR, real-time PCR, mitochondrial DNA

© 2016 Małgorzata Natonek-Wiśniewska et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The use of PCR and Real-Time PCR for Qualitative and Quantitative Determination of Poultry and Chicken Meals

Published Online: Aug 02, 2016

Page range: 731 - 741

Received: Jun 22, 2015

Accepted: Jan 29, 2016

DOI: https://doi.org/10.1515/aoas-2016-0003

Keywordspoultry meal, species identification, PCR, real-time PCR, mitochondrial DNA

© 2016 Małgorzata Natonek-Wiśniewska et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
poultry meal, species identification, PCR, real-time PCR, mitochondrial DNA