<abstract xmlns="http://www.w3.org/1999/xhtml"><p> This study investigated the effects of a 22:6 n-3 rich supplement on ruminal fermentation characteristics and microbial changes, and also the effects of a 22:6 n-3 rich supplement on plasma metabolites by using rumen cannulated wether goats in a 4×4 Latin square design. The 22:6 n-3 rich supplement was infused into the rumen of the goats twice daily in equal portions at a rate of 0.0, 2.5, 5.0 and 10.0 g/d [corresponding to 0, 0.4%, 0.8% and 1.5% of diets (dry material base), respectively]. The concentration of NH<sub>3</sub>-N and total volatile fatty acid (TVFA), and the molar proportions of acetate and butyrate were decreased by the supplement (P&lt;0.001). The supplement decreased diversity of the rumen bacterial community (P&lt;0.01), and reduced the abundance of Ruminococcus flavefaciens, R. albus, Fibrobacter succinogenes and protozoa (P&lt;0.01). However, it increased the abundance of ruminal fungi (P&lt;0.01). The supplement had no influence on the concentration of plasma glucose, HDL-cholesterol, LDL-cholesterol and urea but increased the concentration of triglycerides and total cholesterol (P&lt;0.05). In conclusion, the 22:6 n-3 rich supplement inhibited ruminal fermentation and this was accompanied by the decrease of the abundance of ruminal microbes, and also affected plasma metabolites.</p></abstract>

This study investigated the effects of a 22:6 n-3 rich supplement on ruminal fermentation characteristics and microbial changes, and also the effects of a 22:6 n-3 rich supplement on plasma metabolites by using rumen cannulated wether goats in a 4×4 Latin square design. The 22:6 n-3 rich supplement was infused into the rumen of the goats twice daily in equal portions at a rate of 0.0, 2.5, 5.0 and 10.0 g/d [corresponding to 0, 0.4%, 0.8% and 1.5% of diets (dry material base), respectively]. The concentration of NH3-N and total volatile fatty acid (TVFA), and the molar proportions of acetate and butyrate were decreased by the supplement (P<0.001). The supplement decreased diversity of the rumen bacterial community (P<0.01), and reduced the abundance of Ruminococcus flavefaciens, R. albus, Fibrobacter succinogenes and protozoa (P<0.01). However, it increased the abundance of ruminal fungi (P<0.01). The supplement had no influence on the concentration of plasma glucose, HDL-cholesterol, LDL-cholesterol and urea but increased the concentration of triglycerides and total cholesterol (P<0.05). In conclusion, the 22:6 n-3 rich supplement inhibited ruminal fermentation and this was accompanied by the decrease of the abundance of ruminal microbes, and also affected plasma metabolites.

17. A 22:6 n-3 Rich Supplement Affects the Ruminal Microbial Community and Fermentation and Alters Plasma Metabolites

Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

This study investigated the effects of a 22:6 n-3 rich supplement on ruminal fermentation characteristics and microbial changes, and also the effects of a 22:6...

17. A 22:6 n-3 Rich Supplement Affects the Ruminal Microbial Community and Fermentation and Alters Plasma Metabolites

Article Category: Animal Nutrition, and Feedstuffs

Published Online: May 06, 2016

Page range: 533 - 550

Received: Dec 08, 2014

Accepted: Jan 19, 2016

DOI: https://doi.org/10.1515/aoas-2015-0100

Keywords
22:6 n-3, goat, ruminal fermentation, ruminal microbes

© by Weiyun Zhu

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

17. A 22:6 n-3 Rich Supplement Affects the Ruminal Microbial Community and Fermentation and Alters Plasma Metabolites

Article Category: Animal Nutrition, and Feedstuffs

Published Online: May 06, 2016

Page range: 533 - 550

Received: Dec 08, 2014

Accepted: Jan 19, 2016

DOI: https://doi.org/10.1515/aoas-2015-0100

Keywords22:6 n-3, goat, ruminal fermentation, ruminal microbes

© by Weiyun Zhu

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
22:6 n-3, goat, ruminal fermentation, ruminal microbes