<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Objectives: The purpose of the study was to determine the omega-3/omega-6 ratio in swine brain homogenate by HPLC with UV detection and to discuss the values obtained by comparison to the human species. Materials and methods: Determinations were performed by HPLC method using as mobile phase an isocratic mixture (A:B - 5:95) of mobile phase A = 25% acetonitrile in water and B = acetonitrile with a flow-rate of 1.2 mL/min and UV detection at 205nm. Chromatographic column: Phenomenex C8 150x4.6 mm 5μm. 50 g swine brain was hydrolyzed with 100 mL 0.5N HCl, the organic phase was extracted in 50 mL hexane, concentrated by evaporation and resumed in 200 μL acetonitrile. Results: Polyunsaturated fatty acids were separated as follows arachidonic acid (AA) - Rt = 2.69 min, docosahexaenoic acid (DHA) - Rt = 3.12 min and eicosapentaenoic acid (EPA) - Rt = 3.97 min. The following omega-3/omega-6 ratios were calculated (DHA + EPA)/ AA = 0.572 ± 0.451, EPA/AA = 0.027 ± 0.015 and DHA/AA = 0.689 ± 0.612. Conclusions: The values obtained for these ratios should be balanced, but in reality they are in favor of the ratio denominator. Considering the physiological and nutritional similarities and that an accurate diagnosis of neurodegenerative disease is set in post-mortem, swine brain homogenate could serve as an animal model for human nervous tissue.</p></abstract>

Objectives: The purpose of the study was to determine the omega-3/omega-6 ratio in swine brain homogenate by HPLC with UV detection and to discuss the values obtained by comparison to the human species. Materials and methods: Determinations were performed by HPLC method using as mobile phase an isocratic mixture (A:B - 5:95) of mobile phase A = 25% acetonitrile in water and B = acetonitrile with a flow-rate of 1.2 mL/min and UV detection at 205nm. Chromatographic column: Phenomenex C8 150x4.6 mm 5μm. 50 g swine brain was hydrolyzed with 100 mL 0.5N HCl, the organic phase was extracted in 50 mL hexane, concentrated by evaporation and resumed in 200 μL acetonitrile. Results: Polyunsaturated fatty acids were separated as follows arachidonic acid (AA) - Rt = 2.69 min, docosahexaenoic acid (DHA) - Rt = 3.12 min and eicosapentaenoic acid (EPA) - Rt = 3.97 min. The following omega-3/omega-6 ratios were calculated (DHA + EPA)/ AA = 0.572 ± 0.451, EPA/AA = 0.027 ± 0.015 and DHA/AA = 0.689 ± 0.612. Conclusions: The values obtained for these ratios should be balanced, but in reality they are in favor of the ratio denominator. Considering the physiological and nutritional similarities and that an accurate diagnosis of neurodegenerative disease is set in post-mortem, swine brain homogenate could serve as an animal model for human nervous tissue.

Determination of Omega-3/Omega-6 Ratio in Swine Brain Homogenate as an Animal Model for Human Nervous Tissue

Department of Pharmaceutical Biochemistry, University of Medicine and Pharmacy Tîrgu Mureș, Romania

Department of Pharmacology and Clinical Pharmacy, University of Medicine and Pharmacy Tîrgu Mureș, Romania

Department of Analytical Chemistry and Drug Analysis, University of Medicine and Pharmacy Tîrgu Mureș, Romania

Department of Pharmaceutical Chemistry, University of Medicine and Pharmacy Tîrgu Mureș, Romania

Acta Marisiensis - Seria Medica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Objectives: The purpose of the study was to determine the omega-3/omega-6 ratio in swine brain homogenate by HPLC with UV detection and to discuss the values...

Determination of Omega-3/Omega-6 Ratio in Swine Brain Homogenate as an Animal Model for Human Nervous Tissue

Published Online: Feb 09, 2016

Page range: 86 - 89

Received: Aug 13, 2015

Accepted: Sep 03, 2015

DOI: https://doi.org/10.1515/amma-2015-0093

Keywords
HPLC-UV, eicosapentaenoic acid, docosahexaenoic acid, arachidonic acid, Omega-3 Index

© by Bianca-Eugenia Osz

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Determination of Omega-3/Omega-6 Ratio in Swine Brain Homogenate as an Animal Model for Human Nervous Tissue

Published Online: Feb 09, 2016

Page range: 86 - 89

Received: Aug 13, 2015

Accepted: Sep 03, 2015

DOI: https://doi.org/10.1515/amma-2015-0093

KeywordsHPLC-UV, eicosapentaenoic acid, docosahexaenoic acid, arachidonic acid, Omega-3 Index

© by Bianca-Eugenia Osz

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
HPLC-UV, eicosapentaenoic acid, docosahexaenoic acid, arachidonic acid, Omega-3 Index