<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The present study aims to investigate the NALP3 system and its influence on occludin in Sertoli cells, utilising primary murine cells and adult Sertoli cell line as models. Its main goals are the Sertoli cell biology with possible implications on male reproductive functions. Primary and adult Sertoli cells were transfected with NAPL3 siRNA and treated with NOD1 (ie-DAP) and NOD2 (MDP) receptor ligands. There was positive occludin expression levels on transcript (RT-qPCR) and protein (FCS and Immunofluorescence) levels for both cell types. The innate immunity and tight-junction pathways integration serve a protective role for both testis immune barrier and spermatogenesis compartmentalisation maintained by the very same barrier. This integration also points the way for mechanistic research of the disturbances inflicted during an inflammatory response in testis niche.</p></abstract>

The present study aims to investigate the NALP3 system and its influence on occludin in Sertoli cells, utilising primary murine cells and adult Sertoli cell line as models. Its main goals are the Sertoli cell biology with possible implications on male reproductive functions. Primary and adult Sertoli cells were transfected with NAPL3 siRNA and treated with NOD1 (ie-DAP) and NOD2 (MDP) receptor ligands. There was positive occludin expression levels on transcript (RT-qPCR) and protein (FCS and Immunofluorescence) levels for both cell types. The innate immunity and tight-junction pathways integration serve a protective role for both testis immune barrier and spermatogenesis compartmentalisation maintained by the very same barrier. This integration also points the way for mechanistic research of the disturbances inflicted during an inflammatory response in testis niche.

Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction

Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia, Bulgaria

Acta Medica Bulgarica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The present study aims to investigate the NALP3 system and its influence on occludin in Sertoli cells, utilising primary murine cells and adult Sertoli cell...

Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction

Published Online: May 22, 2015

Page range: 12 - 17

DOI: https://doi.org/10.1515/amb-2015-0002

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.