<abstract xmlns="http://www.w3.org/1999/xhtml"><p> In this study process fluids were tested after the addition of nanoparticles. Cooling and lubricating process fluids are used in machining to reduce wear on tools, to increase machine performance and to improve product quality. The use of process fluids leads to their pollution and contamination. Nanoparticles were added to the process fluids in order to increase their antibacterial activity. The selected nanoparticles were nanoparticles of metallic silver. The process fluids were modified by the addition of silver nitrate and ascorbic acid. Reduction of silver nanoparticles in the volume of the fluid was achieved using UV. The modified fluids were tested for their cytotoxicity and changes in chemical composition. The cytotoxicity of process fluids was tested for the purpose of verifying whether the process fluids, which are in direct contact with the skin of the operator, affect the health of the operator. The cytotoxicity of the process fluids was tested on human fibroblast cells. Fibroblasts are the basic cells of fibrous tissue. The cytotoxicity was tested by measuring the cell viability and using XTT. Analysis of chemical composition was performed for the purpose of determining the individual substances in the process fluids and their chemical stability. Qualitative analysis of the process fluids was performed using gas chromatography mass spectrometry (GC - MS).</p></abstract>

In this study process fluids were tested after the addition of nanoparticles. Cooling and lubricating process fluids are used in machining to reduce wear on tools, to increase machine performance and to improve product quality. The use of process fluids leads to their pollution and contamination. Nanoparticles were added to the process fluids in order to increase their antibacterial activity. The selected nanoparticles were nanoparticles of metallic silver. The process fluids were modified by the addition of silver nitrate and ascorbic acid. Reduction of silver nanoparticles in the volume of the fluid was achieved using UV. The modified fluids were tested for their cytotoxicity and changes in chemical composition. The cytotoxicity of process fluids was tested for the purpose of verifying whether the process fluids, which are in direct contact with the skin of the operator, affect the health of the operator. The cytotoxicity of the process fluids was tested on human fibroblast cells. Fibroblasts are the basic cells of fibrous tissue. The cytotoxicity was tested by measuring the cell viability and using XTT. Analysis of chemical composition was performed for the purpose of determining the individual substances in the process fluids and their chemical stability. Qualitative analysis of the process fluids was performed using gas chromatography mass spectrometry (GC - MS).

The Influence of Nanoadditives on the Biological Properties and Chemical Composition of Process Fluids

Technical University of Liberec, Faculty of textile engineering, Studentská 2, 461 17 Liberec, Czech Rep.

Technical University of Liberec, Inst. for Nanomaterials, Advanced Technology and Innovation, Studentská 2, 461 17 Liberec, Czech Rep.

Advances in Materials Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

In this study process fluids were tested after the addition of nanoparticles. Cooling and lubricating process fluids are used in machining to reduce wear on...

The Influence of Nanoadditives on the Biological Properties and Chemical Composition of Process Fluids

Published Online: Dec 30, 2015

Page range: 59 - 66

DOI: https://doi.org/10.1515/adms-2015-0023

Keywords
nanoparticles, silver, cytotoxicity, chemical composition

© by K. Borůvková

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The Influence of Nanoadditives on the Biological Properties and Chemical Composition of Process Fluids

Published Online: Dec 30, 2015

Page range: 59 - 66

DOI: https://doi.org/10.1515/adms-2015-0023

Keywordsnanoparticles, silver, cytotoxicity, chemical composition

© by K. Borůvková

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
nanoparticles, silver, cytotoxicity, chemical composition