<abstract xmlns="http://www.w3.org/1999/xhtml"><p> This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantifi ed by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our fi ndings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.</p></abstract>

This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantifi ed by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our fi ndings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.

Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide...

Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats

Published Online: Dec 29, 2017

Page range: 540 - 550

Received: Jan 18, 2017

Accepted: May 24, 2017

DOI: https://doi.org/10.1515/acve-2017-0043

Keywords
chronic restraint stress, copper-zinc superoxide dismutase, manganese superoxide dismutase, catalase, hippocampus, rats

© by Nataša Popović

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats

Published Online: Dec 29, 2017

Page range: 540 - 550

Received: Jan 18, 2017

Accepted: May 24, 2017

DOI: https://doi.org/10.1515/acve-2017-0043

Keywordschronic restraint stress, copper-zinc superoxide dismutase, manganese superoxide dismutase, catalase, hippocampus, rats

© by Nataša Popović

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
chronic restraint stress, copper-zinc superoxide dismutase, manganese superoxide dismutase, catalase, hippocampus, rats