<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify <italic>Salmonella enterica</italic> species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 <italic>Salmonella</italic> isolates were identified as <italic>S</italic>. Infantis and <italic>S</italic>. Enteritidis, respectively, while ten isolates were identified as eight different <italic>Salmonella</italic> serovars. Using the same isolates, presence of 993-bp (<italic>bcfC</italic> gene), 636-bp (<italic>steB</italic> gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as <italic>S</italic>. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293-bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as <italic>S</italic>. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of <italic>S</italic>. Infantis, since 25 more isolates were noted as <italic>S</italic>. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as <italic>S</italic>. Typhimurium (1) and <italic>S</italic>. Infantis (6). <italic>S</italic>. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with <italic>S</italic>. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.</p></abstract>

The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293-bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.

Development of PCR-based identification of <italic>Salmonella enterica</italic> serovars

Specialistic veterinary Institute “Subotica”

Laboratory for Microbial Molecular Genetics and Ecology

Institute of Molecular Genetics and Genetic Engineering

Department of Microbiology, Faculty of Veterinary Medicine

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of PCR-based identification of  serovars

The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica...

Development of PCR-based identification of Salmonella enterica serovars

Published Online: Jun 26, 2017

Page range: 271 - 284

Received: Feb 22, 2017

Accepted: May 08, 2017

DOI: https://doi.org/10.1515/acve-2017-0022

Keywords
poultry, multiplex PCR, identification, taxonomy

© 2017 Kiskároly Ferenc et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of PCR-based identification of Salmonella enterica serovars

Published Online: Jun 26, 2017

Page range: 271 - 284

Received: Feb 22, 2017

Accepted: May 08, 2017

DOI: https://doi.org/10.1515/acve-2017-0022

Keywordspoultry, multiplex PCR, identification, taxonomy

© 2017 Kiskároly Ferenc et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
poultry, multiplex PCR, identification, taxonomy