<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Bacillus subtilis sups. chungkookjang</italic> produces a higher molecular mass poly-γ-glutamic acid (γ-PGA). Recently, previous studies have demonstrated immune stimulation and an antitumor effect of the high molecular mass γ-PGA using various mouse models although these effects have not been shown in other species of animals. Therefore, the current study was conducted to determine the effect of γ-PGA in pigs with and without PRRSV infection. PRRS-negative pigs were intramuscularly injected with 1, 3, or 5 ml of 20 mg/m<italic>l</italic>l γ-PGA, and one group of pigs served as a non-treatment (NT) group. All groups treated with γ-PGA had significantly higher weight gains, and pigs treated with 5 ml of γ-PGA exhibited higher tumor necrosis factor (TNF)-α, interferon (IFN)-α and IFN-β expression levels compared with the NT group. According to the preliminary results, an animal challenge study was conducted with a highly virulent PRRSV strain, MN184, along with γ-PGA treatment at different time points. Pigs treated with γ-PGA had lower levels of viral loads in the sera and in lungs and gained significantly more weight (<italic>p</italic>&lt;0.05) compared with the NT group after being challenged with MN184. Moreover, γ-PGA-treatment groups had higher levels of neutralizing antibodies and cytokines related to proinflammatory, humoral and cell-mediated responses than the control group after the PRRSV challenge. Therefore, it was concluded that γ-PGA induces higher levels of immune responses and increases resistance to PRRSV infection in pigs.</p></abstract>

Bacillus subtilis sups. chungkookjang produces a higher molecular mass poly-γ-glutamic acid (γ-PGA). Recently, previous studies have demonstrated immune stimulation and an antitumor effect of the high molecular mass γ-PGA using various mouse models although these effects have not been shown in other species of animals. Therefore, the current study was conducted to determine the effect of γ-PGA in pigs with and without PRRSV infection. PRRS-negative pigs were intramuscularly injected with 1, 3, or 5 ml of 20 mg/mll γ-PGA, and one group of pigs served as a non-treatment (NT) group. All groups treated with γ-PGA had significantly higher weight gains, and pigs treated with 5 ml of γ-PGA exhibited higher tumor necrosis factor (TNF)-α, interferon (IFN)-α and IFN-β expression levels compared with the NT group. According to the preliminary results, an animal challenge study was conducted with a highly virulent PRRSV strain, MN184, along with γ-PGA treatment at different time points. Pigs treated with γ-PGA had lower levels of viral loads in the sera and in lungs and gained significantly more weight (p<0.05) compared with the NT group after being challenged with MN184. Moreover, γ-PGA-treatment groups had higher levels of neutralizing antibodies and cytokines related to proinflammatory, humoral and cell-mediated responses than the control group after the PRRSV challenge. Therefore, it was concluded that γ-PGA induces higher levels of immune responses and increases resistance to PRRSV infection in pigs.

Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection

Corporate Research and Development Center

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Bacillus subtilis sups. chungkookjang produces a higher molecular mass poly-γ-glutamic acid (γ-PGA). Recently, previous studies have demonstrated immune...

Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection

Published Online: Jun 26, 2017

Page range: 153 - 167

Received: Jul 13, 2016

Accepted: Mar 03, 2017

DOI: https://doi.org/10.1515/acve-2017-0014

Keywords
antiviral activity, immune stimulation, neutralizing antibody, poly-γ-glutamic acid, PRRSV

© 2017 Seo Byoung-Joo et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection

Published Online: Jun 26, 2017

Page range: 153 - 167

Received: Jul 13, 2016

Accepted: Mar 03, 2017

DOI: https://doi.org/10.1515/acve-2017-0014

Keywordsantiviral activity, immune stimulation, neutralizing antibody, poly-γ-glutamic acid, PRRSV

© 2017 Seo Byoung-Joo et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
antiviral activity, immune stimulation, neutralizing antibody, poly-γ-glutamic acid, PRRSV