<abstract xmlns="http://www.w3.org/1999/xhtml"> The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples from a total of 200 cattle and 200 sheep were tested and ESBL-producing E. coli was isolated from 31 (15.5%) cattle and three (1.5%) sheep samples using the Clinical and Laboratory Standards Institute’s combined disk method. Among the ESBL gene classes detected by PCR, blaCTX-M was the most frequent type, followed by the blaTEM and blaSHV families. ESBL-producing E. coli isolates showed co-resistance to multiple classes of antibiotics including aminoglycosides, phenicols, quinolones, folate pathway inhibitors and tetracyclines. The resistance rates were higher in the cattle isolates than in the sheep isolates. Phylogenetic grouping of the E. coli isolates indicated group A (particularly A1) was the predominant phylogenetic group (19/34, 55.9%), followed by groups B1 (9/34, 26.5%) and D (6/34, 17.6%); none of the isolates belonged to group B2. The study shows that ESBL-producing E. coli isolates exist in the intestinal flora of healthy cattle and sheep in the Burdur province of Turkey. This is the first report showing the emergence of CTX-M type ESBL-producing E. coli in sheep farms in Turkey</abstract>

The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples from a total of 200 cattle and 200 sheep were tested and ESBL-producing E. coli was isolated from 31 (15.5%) cattle and three (1.5%) sheep samples using the Clinical and Laboratory Standards Institute’s combined disk method. Among the ESBL gene classes detected by PCR, blaCTX-M was the most frequent type, followed by the blaTEM and blaSHV families. ESBL-producing E. coli isolates showed co-resistance to multiple classes of antibiotics including aminoglycosides, phenicols, quinolones, folate pathway inhibitors and tetracyclines. The resistance rates were higher in the cattle isolates than in the sheep isolates. Phylogenetic grouping of the E. coli isolates indicated group A (particularly A1) was the predominant phylogenetic group (19/34, 55.9%), followed by groups B1 (9/34, 26.5%) and D (6/34, 17.6%); none of the isolates belonged to group B2. The study shows that ESBL-producing E. coli isolates exist in the intestinal flora of healthy cattle and sheep in the Burdur province of Turkey. This is the first report showing the emergence of CTX-M type ESBL-producing E. coli in sheep farms in Turkey

Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep

Department of Microbiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur,

Department of Microbiology, Faculty of Veterinary Medicine, Ankara University, Ankara,

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples...

Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep

Published Online: Dec 30, 2016

Page range: 520 - 533

Received: Mar 28, 2016

Accepted: Sep 26, 2016

DOI: https://doi.org/10.1515/acve-2016-0045

Keywords
cattle, ESBL, Escherichia coli, multidrug-resistance, sheep

© by Faruk Pehlivanoglu

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep

Published Online: Dec 30, 2016

Page range: 520 - 533

Received: Mar 28, 2016

Accepted: Sep 26, 2016

DOI: https://doi.org/10.1515/acve-2016-0045

Keywordscattle, ESBL, Escherichia coli, multidrug-resistance, sheep

© by Faruk Pehlivanoglu

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
cattle, ESBL, Escherichia coli, multidrug-resistance, sheep