<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and identification of the existence of resistance genes in resistant strains were conducted. The specimens consist of 600 mastitic milk samples that were collected from 242 cows. Isolation of enterococcus was carried out in selective media and 94 (15.6%) <italic>Enterococcus</italic> spp. were isolated. A total of 94 species of Enterococci were identified using both sequencing and polymerase chain reaction (PCR). <italic>Enterococcus</italic> spp. isolates belong to 5 different species (<italic>E. faecalis, E. faecium, E. durans, E. hirae, E. mundtii</italic>) in sequence analysis and 4 different species (<italic>E. faecalis, E. faecium, E. durans, E. hirae</italic>) were identify by PCR method with specific primers. Analyzing 94 enterococcus strains by antibiotic sensitiveness test a high rate of resistance to tetracycline in 77 (81.9%) isolates was shown. The <italic>tet</italic> resistance genes were identified as follows: 54 were <italic>tet</italic>M positive, 23 were <italic>tet</italic>K positive and 17 were positive on <italic>tet</italic>M and <italic>tet</italic>K. Resistance to erythromycin was established in 27 (28.7%) isolates (25 <italic>erm</italic>B) while the chloramphenicol resistance gene was found in 10 (10.7%) of isolates and the <italic>cat</italic> gene was identified in nine samples and one isolate was resistant to vancomycin (1.06%) with the <italic>Van</italic>A gene confirmed. In conclusion, it was shown that <italic>E. faecalis</italic> has the biggest role in <italic>enterococcus</italic> originated mastitis and these strains were found to be mostly resistant to tetracycline. One vancomycine resistant isolate that had the <italic>Van</italic>A gene was also determined.</p></abstract>

In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and identification of the existence of resistance genes in resistant strains were conducted. The specimens consist of 600 mastitic milk samples that were collected from 242 cows. Isolation of enterococcus was carried out in selective media and 94 (15.6%) Enterococcus spp. were isolated. A total of 94 species of Enterococci were identified using both sequencing and polymerase chain reaction (PCR). Enterococcus spp. isolates belong to 5 different species (E. faecalis, E. faecium, E. durans, E. hirae, E. mundtii) in sequence analysis and 4 different species (E. faecalis, E. faecium, E. durans, E. hirae) were identify by PCR method with specific primers. Analyzing 94 enterococcus strains by antibiotic sensitiveness test a high rate of resistance to tetracycline in 77 (81.9%) isolates was shown. The tet resistance genes were identified as follows: 54 were tetM positive, 23 were tetK positive and 17 were positive on tetM and tetK. Resistance to erythromycin was established in 27 (28.7%) isolates (25 ermB) while the chloramphenicol resistance gene was found in 10 (10.7%) of isolates and the cat gene was identified in nine samples and one isolate was resistant to vancomycin (1.06%) with the VanA gene confirmed. In conclusion, it was shown that E. faecalis has the biggest role in enterococcus originated mastitis and these strains were found to be mostly resistant to tetracycline. One vancomycine resistant isolate that had the VanA gene was also determined.

Distribution of Antibiotic Resistance Genes in <italic>Enterococcus</italic> spp. Isolated from Mastitis Bovine Milk

Veterinary Faculty, Department of Microbiology, Isikli/AYDIN-

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Distribution of Antibiotic Resistance Genes in  spp. Isolated from Mastitis Bovine Milk

In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and...

Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk

Published Online: Sep 29, 2016

Page range: 336 - 346

Received: Jan 05, 2016

Accepted: Apr 26, 2016

DOI: https://doi.org/10.1515/acve-2016-0029

Keywords
Antibiotic resistantance genes, Mastitis, PCR, A

© 2016 Erbas Goksel et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk

Published Online: Sep 29, 2016

Page range: 336 - 346

Received: Jan 05, 2016

Accepted: Apr 26, 2016

DOI: https://doi.org/10.1515/acve-2016-0029

KeywordsAntibiotic resistantance genes, Mastitis, PCR, A

© 2016 Erbas Goksel et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Antibiotic resistantance genes, Mastitis, PCR, A