<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The effect of the probiotic strain <italic>Lactobacillus plantarum</italic> LS/07 on intestinal bacterial enzyme activities – β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in the prevention of cancer, atherosclerosis and dysbiosis was investigated. Male Sprague-Dawley rats were randomly divided into 12 experimental groups: C (control group), AT (atherosclerotic group), CC (carcinogenic group), and then each group in combination with antibiotics and probiotics individually and each group in double combination on antibiotic and probiotic. In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in the atherosclerotic group significantly increased the activity of β-glucuronidase (p&lt;0.001) and β-glucosidase (p&lt;0.01). Azoxymethane application in the carcinogenic group significantly increased β-glucuronidase (p&lt;0.01), but reduced β-glucosidase (p&lt;0.01). Daily application of probiotics individually and in double combination with antibiotics increased the activity of β-galactosidase, and β-glucosidase, and positively decreased the level of β-glucuronidase. In the control antibiotic group β-glucuronidase was significantly increased (p&lt;0.05), and β-glucosidase decreased (p&lt;0.01) which can be caused by a change of microflora in favor of coliform bacteria. These finding indicate the positive effects of probiotic <italic>Lactobacillus plantarum</italic> LS/07 which allows its use in disease prevention in human and veterinary medicine.</p></abstract>

The effect of the probiotic strain Lactobacillus plantarum LS/07 on intestinal bacterial enzyme activities – β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in the prevention of cancer, atherosclerosis and dysbiosis was investigated. Male Sprague-Dawley rats were randomly divided into 12 experimental groups: C (control group), AT (atherosclerotic group), CC (carcinogenic group), and then each group in combination with antibiotics and probiotics individually and each group in double combination on antibiotic and probiotic. In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in the atherosclerotic group significantly increased the activity of β-glucuronidase (p<0.001) and β-glucosidase (p<0.01). Azoxymethane application in the carcinogenic group significantly increased β-glucuronidase (p<0.01), but reduced β-glucosidase (p<0.01). Daily application of probiotics individually and in double combination with antibiotics increased the activity of β-galactosidase, and β-glucosidase, and positively decreased the level of β-glucuronidase. In the control antibiotic group β-glucuronidase was significantly increased (p<0.05), and β-glucosidase decreased (p<0.01) which can be caused by a change of microflora in favor of coliform bacteria. These finding indicate the positive effects of probiotic Lactobacillus plantarum LS/07 which allows its use in disease prevention in human and veterinary medicine.

Effect of <italic>Lactobacillus plantarum</italic> LS/07 on Intestinal Bacterial Enzyme Activities in the Prevention of Cancer, Atherosclerosis and Dysbiosis

Institute of Experimental Medicine, Medical Faculty, University of P. J. Šafárik, Košice,

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of  LS/07 on Intestinal Bacterial Enzyme Activities in the Prevention of Cancer, Atherosclerosis and Dysbiosis

The effect of the probiotic strain Lactobacillus plantarum LS/07 on intestinal bacterial enzyme activities – β-glucuronidase (β-GLUCUR), β-galactosidase...

Effect of Lactobacillus plantarum LS/07 on Intestinal Bacterial Enzyme Activities in the Prevention of Cancer, Atherosclerosis and Dysbiosis

Published Online: Sep 29, 2016

Page range: 294 - 303

Received: Feb 17, 2016

Accepted: Jun 02, 2016

DOI: https://doi.org/10.1515/acve-2016-0026

Keywords
Sprague-Dawley rats, Bacterial enzyme, Cancer, Atherosclerosis, Dysbiosis

© 2016 Hijová Emília et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of Lactobacillus plantarum LS/07 on Intestinal Bacterial Enzyme Activities in the Prevention of Cancer, Atherosclerosis and Dysbiosis

Published Online: Sep 29, 2016

Page range: 294 - 303

Received: Feb 17, 2016

Accepted: Jun 02, 2016

DOI: https://doi.org/10.1515/acve-2016-0026

KeywordsSprague-Dawley rats, Bacterial enzyme, Cancer, Atherosclerosis, Dysbiosis

© 2016 Hijová Emília et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Sprague-Dawley rats, Bacterial enzyme, Cancer, Atherosclerosis, Dysbiosis