<abstract xmlns="http://www.w3.org/1999/xhtml"> Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the morphological differences of hair fibres between two species (dog, sheep) and their ultimate impact on sample collection and processing. Our preliminary findings suggest that the use of EZ1® DNA Investigator Kit yields donor DNA profiles of higher quality. The results of different sample collection methods have shown intraspecific variations that require further investigation. The ability of retention and subsequent extraction of trace DNA appears to be similar between the two species, despite significant morphological differences between their coat hairs.</abstract>

Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the morphological differences of hair fibres between two species (dog, sheep) and their ultimate impact on sample collection and processing. Our preliminary findings suggest that the use of EZ1® DNA Investigator Kit yields donor DNA profiles of higher quality. The results of different sample collection methods have shown intraspecific variations that require further investigation. The ability of retention and subsequent extraction of trace DNA appears to be similar between the two species, despite significant morphological differences between their coat hairs.

Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs

Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

University Department for Forensic Sciences, University of Split, Split, Croatia

Student of doctoral study of veterinary sciences of Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Forensic Science Centre „Ivan Vučetić“, General Police Directorate, Ministry of Interior, Zagreb, Croatia

Student of integrated undergraduate and graduate studies of Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs"}

Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of...

Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs

Published Online: Jun 28, 2016

Page range: 187 - 202

Received: Aug 24, 2015

Accepted: Feb 26, 2016

DOI: https://doi.org/10.1515/acve-2016-0016

Keywords
DNA profiling, sheep wool, dog hair, Chelex® 100, Qiagen EZ1® DNA Investigator Kit

© by Gordan Mršić

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs

Published Online: Jun 28, 2016

Page range: 187 - 202

Received: Aug 24, 2015

Accepted: Feb 26, 2016

DOI: https://doi.org/10.1515/acve-2016-0016

KeywordsDNA profiling, sheep wool, dog hair, Chelex® 100, Qiagen EZ1® DNA Investigator Kit

© by Gordan Mršić

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
DNA profiling, sheep wool, dog hair, Chelex® 100, Qiagen EZ1® DNA Investigator Kit