<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log<sub>2</sub>. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.</p></abstract>

The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.

Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia

Scientific Veterinary Institute „Novi Sad“, Rumenački put 20, 21000 Novi Sad, Republic of Serbia

Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Beograd, Republic of Serbia

Veterinary Pharmacy „Pet Svet 024“, Joa Lajoš 5, 24000 Subotica, Republic of Serbia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

UTVRĐIVANJE VIRUSA VIRUSNOG ARTERITISA U SEMENU PASTUVA U REPUBLICI SRBIJI

The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus...

Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia

Published Online: Dec 22, 2015

Page range: 557 - 567

Received: May 05, 2015

Accepted: Oct 12, 2015

DOI: https://doi.org/10.1515/acve-2015-0047

Keywords
breeding stallions, semen, Equine arteritis virus, RT-PCR, seroprevalence, virus neutralization

© 2015 Lazić Sava et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia

Published Online: Dec 22, 2015

Page range: 557 - 567

Received: May 05, 2015

Accepted: Oct 12, 2015

DOI: https://doi.org/10.1515/acve-2015-0047

Keywordsbreeding stallions, semen, Equine arteritis virus, RT-PCR, seroprevalence, virus neutralization

© 2015 Lazić Sava et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
breeding stallions, semen, Equine arteritis virus, RT-PCR, seroprevalence, virus neutralization