<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of this study was to describe the prevalence of antibiotic resistance to beta-lactams and to evaluate two resistance genes <italic>bla</italic>TEM and <italic>bla</italic>OXA-1 in <italic>Escherichia coli</italic> isolates from faeces on six swine farms in the Republic of Bulgaria. A total of 186 <italic>E. coli</italic> isolates from 192 faecal swabs were tested by the disk diffusion method to determine resistance patterns to 11 antimicrobial agents. Resistance to beta-lactams was determined by disk diffusion method, E-test, micro-broth dilution method and PCR. About 40.3% of the <italic>E. coli</italic> isolates from swine were resistant to ampicillin. The highest resistance was observed in <italic>E.coli</italic> isolates from weaned pigs to ampicillin – 60.0% and to cephalotin – 45.5 %. The <italic>E. coli</italic> isolates resistant to beta-lactams were examined for the presence of <italic>bla</italic>TEM and <italic>bla</italic>OXA-1 genes. The most common <italic>bla</italic> gene identified was <italic>bla</italic>TEM, which was found in 92.0% of swine isolates.</abstract>

The aim of this study was to describe the prevalence of antibiotic resistance to beta-lactams and to evaluate two resistance genes blaTEM and blaOXA-1 in Escherichia coli isolates from faeces on six swine farms in the Republic of Bulgaria. A total of 186 E. coli isolates from 192 faecal swabs were tested by the disk diffusion method to determine resistance patterns to 11 antimicrobial agents. Resistance to beta-lactams was determined by disk diffusion method, E-test, micro-broth dilution method and PCR. About 40.3% of the E. coli isolates from swine were resistant to ampicillin. The highest resistance was observed in E.coli isolates from weaned pigs to ampicillin – 60.0% and to cephalotin – 45.5 %. The E. coli isolates resistant to beta-lactams were examined for the presence of blaTEM and blaOXA-1 genes. The most common bla gene identified was blaTEM, which was found in 92.0% of swine isolates.

Investigations On The Resistance Of Porcine Commensal <italic>E. Coli</italic> Isolates To Beta Lactams

Department of Veterinary Microbiology, Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Student campus, Bulgaria

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigations On The Resistance Of Porcine Commensal  Isolates To Beta Lactams

The aim of this study was to describe the prevalence of antibiotic resistance to beta-lactams and to evaluate two resistance genes blaTEM and blaOXA-1 in...

Investigations On The Resistance Of Porcine Commensal E. Coli Isolates To Beta Lactams

Published Online: Dec 22, 2015

Page range: 538 - 547

Received: Feb 27, 2015

Accepted: May 27, 2015

DOI: https://doi.org/10.1515/acve-2015-0045

Keywords
commensal, ESBL, pigs, resistance to beta-lactams

© 2015 Urumova Valentina, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigations On The Resistance Of Porcine Commensal E. Coli Isolates To Beta Lactams

Published Online: Dec 22, 2015

Page range: 538 - 547

Received: Feb 27, 2015

Accepted: May 27, 2015

DOI: https://doi.org/10.1515/acve-2015-0045

Keywordscommensal, ESBL, pigs, resistance to beta-lactams

© 2015 Urumova Valentina, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
commensal, ESBL, pigs, resistance to beta-lactams