<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 ± 0.9 years) were expanded (37°C, 5% CO<sub>2</sub>, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined <italic>in vitro</italic>. In early passages PTD was 31 ± 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 ± 134. At P1, 0.55 ± 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets’ slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells <italic>in vitro</italic> exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.</p></abstract>

The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 ± 0.9 years) were expanded (37°C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 ± 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 ± 134. At P1, 0.55 ± 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets’ slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.

Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobođenja 18, 11000 Belgrade, Serbia

Institute for Medical Research, University of Belgrade, Serbia, Bul. Oslobođenja 18, 11000 Belgrade, Serbia

INSERM U791, LIOAD, University of Nantes, France, 1-Place Alexis Ricordeau, 44042, Nantes Cedex 1, France

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

POTENCIJAL ZA PROLIFERACIJU I DIFERENCIJACIJU ĆELIJA SINOVIJALNE TEČNOSTI PASA

The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their...

Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

Published Online: Mar 20, 2015

Page range: 66 - 78

Received: Jul 09, 2014

Accepted: Dec 22, 2014

DOI: https://doi.org/10.1515/acve-2015-0005

Keywords
chondrogenesis, dog, mesenchymal stem cell, synovial fluid

© FRANCUSKI V Jelena, DEBELJAK MARTAČIĆ Jasmina, RADOVANOVIĆ Anita, ANDRIĆ Nenad, SOURICE-PETIT Sophie, GUICHEUX Jerome, MOJSILOVIĆ Slavko, KOVAČEVIĆ FILIPOVIĆ Milica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

Published Online: Mar 20, 2015

Page range: 66 - 78

Received: Jul 09, 2014

Accepted: Dec 22, 2014

DOI: https://doi.org/10.1515/acve-2015-0005

Keywordschondrogenesis, dog, mesenchymal stem cell, synovial fluid

© FRANCUSKI V Jelena, DEBELJAK MARTAČIĆ Jasmina, RADOVANOVIĆ Anita, ANDRIĆ Nenad, SOURICE-PETIT Sophie, GUICHEUX Jerome, MOJSILOVIĆ Slavko, KOVAČEVIĆ FILIPOVIĆ Milica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
chondrogenesis, dog, mesenchymal stem cell, synovial fluid