<abstract xmlns="http://www.w3.org/1999/xhtml"><p> HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (R<sub>s</sub> > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.</p></abstract>

HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (Rs > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study,...

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins

Article Category: Original research paper

Published Online: Jun 01, 2017

Page range: 203 - 213

Accepted: Dec 22, 2016

DOI: https://doi.org/10.1515/acph-2017-0013

Keywords
monolithic silica column, fused core column, HPLC, fat-soluble vitamins, analytical performance

© by Sami El Deeb

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins

Article Category: Original research paper

Published Online: Jun 01, 2017

Page range: 203 - 213

Accepted: Dec 22, 2016

DOI: https://doi.org/10.1515/acph-2017-0013

Keywordsmonolithic silica column, fused core column, HPLC, fat-soluble vitamins, analytical performance

© by Sami El Deeb

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
monolithic silica column, fused core column, HPLC, fat-soluble vitamins, analytical performance