<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Centella asiatica</italic> was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-<italic>β</italic>-cyclodextrin (HP-<italic>β</italic>-CD) and formulated with Eudragit E100, glycerol, PEG 400, copovidone, ethanol and purified water. A clear yellowish solution (F1-F8) was obtained. The formulations had a pH of 5.5–6.0 with viscosity in the range of 20–60 mPa s, surface tension 20.3–24.6 mN m<sup>–1</sup> and contact angle less than 20°. The amount of PEG 400 and copovidone affected the film and spreadability. The content of triterpenes in the spray formulation was close to 100 % compared to triterpenes in the extract. The skin irritation study indicated that the formulation was non-irritating in a rat model. An <italic>in vivo</italic> excision wound healing model showed that wound excision was completely healed after 14 days.</p></abstract>

Centella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-β-cyclodextrin (HP-β-CD) and formulated with Eudragit E100, glycerol, PEG 400, copovidone, ethanol and purified water. A clear yellowish solution (F1-F8) was obtained. The formulations had a pH of 5.5–6.0 with viscosity in the range of 20–60 mPa s, surface tension 20.3–24.6 mN m–1 and contact angle less than 20°. The amount of PEG 400 and copovidone affected the film and spreadability. The content of triterpenes in the spray formulation was close to 100 % compared to triterpenes in the extract. The skin irritation study indicated that the formulation was non-irritating in a rat model. An in vivo excision wound healing model showed that wound excision was completely healed after 14 days.

Evaluation of the topical spray containing <italic>Centella asiatica</italic> extract and its efficacy on excision wounds in rats

School of Pharmacy, Walailak University, Thasala District, Nakhon, Si Thammarat, 80161, Thailand

Drug and Cosmetic Research and Development Unit, School of Pharmacy, Walailak University, Thasala District, Nakhon Si Thammarat, 80161, Thailand

Faculty of Public Health, Naresuan University, Tha Pho, Muang, Phitsanulok, 65000, Thailand

Department of Pharmaceutical, Technology and Nanotec-PSU, Excellence Center on Drug Delivery System, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Evaluation of the topical spray containing  extract and its efficacy on excision wounds in rats

Centella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 %...

Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats

Published Online: May 28, 2016

Page range: 233 - 244

Accepted: Jan 20, 2016

DOI: https://doi.org/10.1515/acph-2016-0018

Keywords
extract, hydroxypropyl--cyclodextrin, wound healing, topical spray

© 2016 Somchai Sawatdee et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats

Published Online: May 28, 2016

Page range: 233 - 244

Accepted: Jan 20, 2016

DOI: https://doi.org/10.1515/acph-2016-0018

Keywordsextract, hydroxypropyl--cyclodextrin, wound healing, topical spray

© 2016 Somchai Sawatdee et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
extract, hydroxypropyl--cyclodextrin, wound healing, topical spray