<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg<sup>-1</sup>, was highly effective in increasing nitric oxide production. At the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical.</p></abstract>

Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg-1, was highly effective in increasing nitric oxide production. At the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical.

Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo

Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş Tîrgu Mureş 540138, Romania

Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University Cluj-Napoca 400028, Romania

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in...

Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo

Published Online: Dec 17, 2015

Page range: 413 - 426

Accepted: Jul 13, 2015

DOI: https://doi.org/10.1515/acph-2015-0032

Keywords
nitric oxide, nitrite, nitrate, nitrones, nitric oxide donor

© by Ibolya Fülöp

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo

Published Online: Dec 17, 2015

Page range: 413 - 426

Accepted: Jul 13, 2015

DOI: https://doi.org/10.1515/acph-2015-0032

Keywordsnitric oxide, nitrite, nitrate, nitrones, nitric oxide donor

© by Ibolya Fülöp

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
nitric oxide, nitrite, nitrate, nitrones, nitric oxide donor