<abstract xmlns="http://www.w3.org/1999/xhtml"><sec><h3>Background</h3><p>Nuclear protein in testis (NUT) midline carcinoma (NMC) is an undifferentiated carcinoma, usually localized to the midline and presenting a translocation in the gene for bromodomain containing protein 4. Here, we report a rare case of NMC in an 8-year-old Turkish boy.</p></sec><sec><h3>Case report</h3><p>There were masses in the lung, liver, and iliac wing representing metastases. Abdominal lymph node sampling revealed epithelial tumor infiltration with cellular pleomorphism. Immunohistochemistry was strongly positive for cytokeratin and epithelial membrane antigen protein. Because of undifferentiated carcinoma morphology, the tumor was considered to be a NMC. Immunoreactivity with antibodies to NUT and the presence of NUT clarified by fluorescence in situ hybridization (FISH) supported the diagnosis. Despite initial response to chemotherapy, the patient died 7 months after the diagnosis.</p></sec><sec><h3>Conclusions</h3><p>Immunoreactivity for NUT antibodies along with a dual-color FISH and karyotype analysis was suggestive for diagnosis of NMC. In differential diagnosis of undifferentiated carcinomas that occur particularly at midline localization, NMC should be considered.</p></sec></abstract>

BackgroundNuclear protein in testis (NUT) midline carcinoma (NMC) is an undifferentiated carcinoma, usually localized to the midline and presenting a translocation in the gene for bromodomain containing protein 4. Here, we report a rare case of NMC in an 8-year-old Turkish boy.Case reportThere were masses in the lung, liver, and iliac wing representing metastases. Abdominal lymph node sampling revealed epithelial tumor infiltration with cellular pleomorphism. Immunohistochemistry was strongly positive for cytokeratin and epithelial membrane antigen protein. Because of undifferentiated carcinoma morphology, the tumor was considered to be a NMC. Immunoreactivity with antibodies to NUT and the presence of NUT clarified by fluorescence in situ hybridization (FISH) supported the diagnosis. Despite initial response to chemotherapy, the patient died 7 months after the diagnosis.ConclusionsImmunoreactivity for NUT antibodies along with a dual-color FISH and karyotype analysis was suggestive for diagnosis of NMC. In differential diagnosis of undifferentiated carcinomas that occur particularly at midline localization, NMC should be considered.

Nuclear protein in testis midline carcinoma in a Turkish boy: a case report

Department of Pathology, Faculty of Medicine

Department of Pediatric Oncology, Faculty of Medicine

Department of Radiology, Faculty of Medicine

Department of Pathology, Harvard Medical School

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Nuclear protein in testis midline carcinoma in a Turkish boy: a case report"}

BackgroundNuclear protein in testis (NUT) midline carcinoma (NMC) is an undifferentiated carcinoma, usually localized to the midline and presenting a...

Nuclear protein in testis midline carcinoma in a Turkish boy: a case report

Article Category: Clinical report

Published Online: Oct 31, 2020

Page range: 203 - 208

DOI: https://doi.org/10.1515/abm-2020-0029

Keywords
carcinoma, immunohistochemistry, in situ hybridization, fluorescence, nuclear protein in testis, human, Turkey

© 2020 Büşra Yaprak Bayrak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Nuclear protein in testis midline carcinoma in a Turkish boy: a case report

Article Category: Clinical report

Published Online: Oct 31, 2020

Page range: 203 - 208

DOI: https://doi.org/10.1515/abm-2020-0029

Keywordscarcinoma, immunohistochemistry, in situ hybridization, fluorescence, nuclear protein in testis, human, Turkey

© 2020 Büşra Yaprak Bayrak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
carcinoma, immunohistochemistry, in situ hybridization, fluorescence, nuclear protein in testis, human, Turkey