<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_abm-2019-0041_s_004_w2aab2b8b5b1b7b1aab1c14b1Aa"><h3>Background</h3><p>Previous studies showed that clinically significant pathogens were detected within 3 days of incubation using a continuous monitoring automated blood culture instrument.</p></sec><sec id="j_abm-2019-0041_s_005_w2aab2b8b5b1b7b1aab1c14b2Aa"><h3>Objectives</h3><p>To determine time to blood culture positivity (TTP) of microorganisms using a BD BACTEC™ FX.</p></sec><sec id="j_abm-2019-0041_s_006_w2aab2b8b5b1b7b1aab1c14b3Aa"><h3>Methods</h3><p>A cross-sectional study was conducted at Maharaj Nakorn Chiang Mai Hospital, Thailand from October to November 2014.</p></sec><sec id="j_abm-2019-0041_s_007_w2aab2b8b5b1b7b1aab1c14b4Aa"><h3>Results</h3><p>One-hundred and eighty-one patients with 195 episodes of infection and 436 cultures were included in the analysis. Among 181 patients, 55.2% were male and the median age was 61 years (interquartile range (IQR) 50, 76). Of the 195 episodes of infections, the most common source was genitourinary tract (15.4%). Overall, the median TTP was 17 hours (IQR 11.5, 24.5), the shortest TTP was observed in <italic>Streptococcus agalactiae</italic>. Four-hundred and seventy-eight cultures (97.6%) and all (100%) were detected at 3 days and 5 days of incubation. Factors associated with TTP ≤24 hour were blood drawn from patients who had hematologic malignancy (odds ratio (OR) 9.6, 95% confidence interval (CI) 1.2, 74.3, <italic>P</italic> = 0.030), endocarditis and vascular infection (OR 8.7, 95% CI 1.1, 67.2, <italic>P</italic> = 0.038), thrombocytopenia (OR 2.4, 95% CI 1.3, 4.4, <italic>P</italic> = 0.004), clinical of systemic inflammatory response syndrome (SIRS) (OR 2.3, 95% CI 1.2, 4.5, <italic>P</italic> = 0.014), and not receiving antimicrobials within 72 hours before cultures taken (OR 2.2, 95% CI 1.4, 3.6, <italic>P</italic> &lt; 0.001).</p></sec><sec id="j_abm-2019-0041_s_008_w2aab2b8b5b1b7b1aab1c14b5Aa"><h3>Conclusions</h3><p>TTP varied depends upon the pathogens and clinical settings. However, bacteria were isolated from almost, but not all of the blood cultures within 3 days of incubation.</p></sec></abstract>

BackgroundPrevious studies showed that clinically significant pathogens were detected within 3 days of incubation using a continuous monitoring automated blood culture instrument.ObjectivesTo determine time to blood culture positivity (TTP) of microorganisms using a BD BACTEC™ FX.MethodsA cross-sectional study was conducted at Maharaj Nakorn Chiang Mai Hospital, Thailand from October to November 2014.ResultsOne-hundred and eighty-one patients with 195 episodes of infection and 436 cultures were included in the analysis. Among 181 patients, 55.2% were male and the median age was 61 years (interquartile range (IQR) 50, 76). Of the 195 episodes of infections, the most common source was genitourinary tract (15.4%). Overall, the median TTP was 17 hours (IQR 11.5, 24.5), the shortest TTP was observed in Streptococcus agalactiae. Four-hundred and seventy-eight cultures (97.6%) and all (100%) were detected at 3 days and 5 days of incubation. Factors associated with TTP ≤24 hour were blood drawn from patients who had hematologic malignancy (odds ratio (OR) 9.6, 95% confidence interval (CI) 1.2, 74.3, P = 0.030), endocarditis and vascular infection (OR 8.7, 95% CI 1.1, 67.2, P = 0.038), thrombocytopenia (OR 2.4, 95% CI 1.3, 4.4, P = 0.004), clinical of systemic inflammatory response syndrome (SIRS) (OR 2.3, 95% CI 1.2, 4.5, P = 0.014), and not receiving antimicrobials within 72 hours before cultures taken (OR 2.2, 95% CI 1.4, 3.6, P < 0.001).ConclusionsTTP varied depends upon the pathogens and clinical settings. However, bacteria were isolated from almost, but not all of the blood cultures within 3 days of incubation.

Time to blood cultures positivity of microorganisms using a continuous-monitoring automated blood cultures system

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

BackgroundPrevious studies showed that clinically significant pathogens were detected within 3 days of incubation using a continuous monitoring automated blood...

Time to blood cultures positivity of microorganisms using a continuous-monitoring automated blood cultures system

Article Category: Original article

Published Online: Dec 19, 2019

Page range: 61 - 69

DOI: https://doi.org/10.1515/abm-2019-0041

Keywords
bacteremia, blood culture, fungemia

© 2019 Krisanapan and Chaiwarith, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Time to blood cultures positivity of microorganisms using a continuous-monitoring automated blood cultures system

Article Category: Original article

Published Online: Dec 19, 2019

Page range: 61 - 69

DOI: https://doi.org/10.1515/abm-2019-0041

Keywordsbacteremia, blood culture, fungemia

© 2019 Krisanapan and Chaiwarith, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
bacteremia, blood culture, fungemia