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Changes in protein patterns of Staphylococcus aureus and Escherichia coli by silver nanoparticles capped with poly (4-styrenesulfonic acid-co-maleic acid) polymer

Hathaichanok Tamiyakul
Hathaichanok Tamiyakul
, 
Sittiruk Roytrakul
Sittiruk Roytrakul
, 
Janthima Jaresitthikunchai
Janthima Jaresitthikunchai
, 
Narumon Phaonakrop
Narumon Phaonakrop
, 
Somboon Tanasupawat
Somboon Tanasupawat
 and 
Warangkana Warisnoicharoen
Warangkana Warisnoicharoen
   | Dec 19, 2019
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Article Category: Original article
Published Online: Dec 19, 2019
Page range: 39 - 47
DOI: https://doi.org/10.1515/abm-2019-0039
Keywords
© 2019 Hathaichanok Tamiyakul et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

(A) Growth curves of Staphylococcus aureus ATCC 25923 cells and (B) those of Escherichia coli ATCC 25922 cells after exposed (solid line) and unexposed (dashed line) to nanosilver. Error bars indicate standard deviations of the means of n = 4. (C) Patterns of total protein extracted from S. aureus ATCC 25923 and (D) those of E. coli ATCC 25922 after (left) unexposed and (right) exposed to nanosilver at different incubation times (a: 0 min, b: 15 min, c: 30 min, d: 45 min, e: 60 min, f: 120 min, and g: 180 min).
(A) Growth curves of Staphylococcus aureus ATCC 25923 cells and (B) those of Escherichia coli ATCC 25922 cells after exposed (solid line) and unexposed (dashed line) to nanosilver. Error bars indicate standard deviations of the means of n = 4. (C) Patterns of total protein extracted from S. aureus ATCC 25923 and (D) those of E. coli ATCC 25922 after (left) unexposed and (right) exposed to nanosilver at different incubation times (a: 0 min, b: 15 min, c: 30 min, d: 45 min, e: 60 min, f: 120 min, and g: 180 min).

Figure 2

Interaction network of matched categories for (left) Staphylococcus aureus ATCC 25923 and (right) Escherichia coli ATCC 25922. Thicker lines indicate higher confidence and higher number of interactions described by String database.
Interaction network of matched categories for (left) Staphylococcus aureus ATCC 25923 and (right) Escherichia coli ATCC 25922. Thicker lines indicate higher confidence and higher number of interactions described by String database.

The identified proteins by LC–MS/MS with known functions of Staphylococcus aureus and Escherichia coli with untreated (control) and nanosilver (NS)-treated conditions

Protein number accessionProtein nameProtein symbolFunction
S. aureus (control)
gi|170081960Long-chain fatty acid transport proteinfadLMolecular transport
S. aureus (NS treated)
gi|3267984693-Deoxy-d-manno-octulosonic-acid transferasewaaAMetabolic process
S. aureus (control and NS treated)
gi|17869702,3-Bisphosphoglycerate-dependent phosphoglycerate mutasegpmAMetabolic process
E. coli (control)
gi|1786964Quinolinate synthase AnadAMetabolic process
gi|153844091Protein YhgFyhgFMetabolic process
E. coli (NS treated)
gi|146319043Threonine dehydrogenase and related Zn-dependent dehydrogenasesrlBMetabolic process
gi|308050024Flagellar hook-basal body proteinfliEMetabolic process
gi|156973219UDP-N-acetylmuramoyl-l-alanyl-d-glutamate synthetasemurDMetabolic process
gi|1279404Peptide transport system ATP-binding protein SapFsapFMolecular transport
gi|323524861Enoyl-CoA hydratase/isomerasetdcFSignal transduction
gi|1787018Probable ATP-dependent helicase DinGdinGDNA replication
gi|283778629DNA topoisomerase type IA Zn finger domain-containing proteinyrdDTranscription
gi|178971550S ribosomal protein L4rplDTranslation
E. coli (control and NS treated)
gi|227357988Siderophore biosynthesis IucA/IucC family proteiniucAMetabolic process
gi|226943115Isopropylmalate isomerase large subunitleuCMetabolic process
gi|225010785PhoH family proteinphoHMetabolic process
gi|261854682Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolasepurHMetabolic process
gi|319762019Haloacid dehalogenase (HAD)-superfamily hydrolaseyqaBMetabolic process
gi|331007050Colicin V production proteincvpAMolecular transport
gi|325067458Oligopeptide ATP-biding cassette (ABC) transporter periplasmic proteinoppAMolecular transport
gi|320353100OmpA/MotB domain-containing proteinmotBMolecular transport
gi|257459944Putative TonB-dependent receptortonBSignal transduction
gi|333984533TonB-dependent siderophore receptoryncDSignal transduction
gi|312196358IclR family transcriptional regulatoriclRTranscription
gi|384412003NusA antitermination factornusATranscription
gi|85860692ATP-dependent RNA helicaserhlBTranscription
gi|260893293Alanyl-tRNA synthetasealaSTranslation
gi|227495901Leucine—tRNA ligaseleuSTranslation
gi|2965357950S ribosomal protein L1rplATranslation
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