<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_abm-2018-0029_s_004_w2aab3b7b4b1b6b1aab1c16b1Aa"><h3>Background</h3><p>Hydroxyapatite is widely used as a coating on metallic implants to promote bioactivity. The coating is typically produced using a high temperature, resulting in phase heterogeneity and coating delamination, which may lead to failure of the coating clinically. Development of a simple and low-temperature hydroxyapatite coating technique may improve the bone bonding ability of implants.</p></sec><sec id="j_abm-2018-0029_s_005_w2aab3b7b4b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To investigate responses to hydroxyapatite-coated titanium produced by a newly developed sol–gel by osteoblasts in vitro and bone in vivo.</p></sec><sec id="j_abm-2018-0029_s_006_w2aab3b7b4b1b6b1aab1c16b3Aa"><h3>Methods</h3><p>Osteoblast proliferation was characterized using a methyl thiazolyl tetrazolium assay and cell calcification with an Alizarin red S assay, and the results were compared with those of uncoated titanium. Uncoated and coated screws were inserted into the trabecular bone of New Zealand white rabbit legs. These implants were evaluated mechanically and histologically after 7, 12, and 24 weeks.</p></sec><sec id="j_abm-2018-0029_s_007_w2aab3b7b4b1b6b1aab1c16b4Aa"><h3>Results</h3><p>Hydroxyapatite-coated titanium showed a significantly greater cell proliferation and mineralization than uncoated titanium. Extraction torques for the coated screws increased with time of implantation and were significantly greater than those of uncoated screws. We observed bone fragments attached to the surface of all coated screws after removal, but none on uncoated screws. Hematoxylin and eosin-stained bone showed no active inflammatory responses to implantation at any time examined. Bone surrounding either uncoated or coated screws followed typical remodeling stages, but maturation of bone healing was faster with coated screws.</p></sec><sec id="j_abm-2018-0029_s_008_w2aab3b7b4b1b6b1aab1c16b5Aa"><h3>Conclusions</h3><p>The sol–gel-derived hydroxyapatite coating showed bioactivity, indicating its potential application as an alternative coating technique to improve the bone bonding ability of implants.</p></sec></abstract>

BackgroundHydroxyapatite is widely used as a coating on metallic implants to promote bioactivity. The coating is typically produced using a high temperature, resulting in phase heterogeneity and coating delamination, which may lead to failure of the coating clinically. Development of a simple and low-temperature hydroxyapatite coating technique may improve the bone bonding ability of implants.ObjectivesTo investigate responses to hydroxyapatite-coated titanium produced by a newly developed sol–gel by osteoblasts in vitro and bone in vivo.MethodsOsteoblast proliferation was characterized using a methyl thiazolyl tetrazolium assay and cell calcification with an Alizarin red S assay, and the results were compared with those of uncoated titanium. Uncoated and coated screws were inserted into the trabecular bone of New Zealand white rabbit legs. These implants were evaluated mechanically and histologically after 7, 12, and 24 weeks.ResultsHydroxyapatite-coated titanium showed a significantly greater cell proliferation and mineralization than uncoated titanium. Extraction torques for the coated screws increased with time of implantation and were significantly greater than those of uncoated screws. We observed bone fragments attached to the surface of all coated screws after removal, but none on uncoated screws. Hematoxylin and eosin-stained bone showed no active inflammatory responses to implantation at any time examined. Bone surrounding either uncoated or coated screws followed typical remodeling stages, but maturation of bone healing was faster with coated screws.ConclusionsThe sol–gel-derived hydroxyapatite coating showed bioactivity, indicating its potential application as an alternative coating technique to improve the bone bonding ability of implants.

Bioactivity of a sol–gel-derived hydroxyapatite coating on titanium implants in vitro and in vivo

National Metal and Materials Technology Center

National Science and Technology Development Agency, Ministry of Science and Technology

Department of Orthopaedics, Faculty of Medicine

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

BackgroundHydroxyapatite is widely used as a coating on metallic implants to promote bioactivity. The coating is typically produced using a high temperature,...

Bioactivity of a sol–gel-derived hydroxyapatite coating on titanium implants in vitro and in vivo

Article Category: Brief communication (original)

Published Online: Dec 31, 2018

Page range: 35 - 44

DOI: https://doi.org/10.1515/abm-2018-0029

Keywords
bone–implant interface, bone remodeling, hydroxyapatites, osseointegration, sol–gel process

© 2018 Jintamai Suwanprateeb et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Bioactivity of a sol–gel-derived hydroxyapatite coating on titanium implants in vitro and in vivo

Article Category: Brief communication (original)

Published Online: Dec 31, 2018

Page range: 35 - 44

DOI: https://doi.org/10.1515/abm-2018-0029

Keywordsbone–implant interface, bone remodeling, hydroxyapatites, osseointegration, sol–gel process

© 2018 Jintamai Suwanprateeb et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
bone–implant interface, bone remodeling, hydroxyapatites, osseointegration, sol–gel process