<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of this study was to test the effect of extenders containing different sugar in their composition on ram sperm motility and pregnancy rate of ewe’s following artificial insemination. Semen were collected from ten North-east Bulgarian fine-fleece breed and tested for quality. Semen was diluted with different extenders, with di- and trisaccharides. A series of experiments were repeated in triplicate. Total motility was determined by using Sperm Analysis (SCA, Microptic, Spain). A total of 200 North-east Bulgarian fine-fleece breed mature ewes were used for cervical insemination with a sperm dose at the concentration of 100 × 10<sup>6</sup> spermatozoa. Pregnancies were diagnosed 60 days after AI by - a real-time ultrasonic scan device (Alloka SSD 500). In conclusion, our experiments demonstrated that higher sperm motility after storage at 4°C for 24 hours and 48 hours has a ram spermatozoa diluted with extender 1, with combination of disaccharides (sucrose and lactose) and trisaccharides (rafinosa). This semen extender (number 1) can be used for successful insemination of ewes and to enhance pregnancy rate after artificial insemination.</p></abstract>

The aim of this study was to test the effect of extenders containing different sugar in their composition on ram sperm motility and pregnancy rate of ewe’s following artificial insemination. Semen were collected from ten North-east Bulgarian fine-fleece breed and tested for quality. Semen was diluted with different extenders, with di- and trisaccharides. A series of experiments were repeated in triplicate. Total motility was determined by using Sperm Analysis (SCA, Microptic, Spain). A total of 200 North-east Bulgarian fine-fleece breed mature ewes were used for cervical insemination with a sperm dose at the concentration of 100 × 106 spermatozoa. Pregnancies were diagnosed 60 days after AI by - a real-time ultrasonic scan device (Alloka SSD 500). In conclusion, our experiments demonstrated that higher sperm motility after storage at 4°C for 24 hours and 48 hours has a ram spermatozoa diluted with extender 1, with combination of disaccharides (sucrose and lactose) and trisaccharides (rafinosa). This semen extender (number 1) can be used for successful insemination of ewes and to enhance pregnancy rate after artificial insemination.

Effect of Different Extenders and Storage Periods on Motility and Fertility of Ram Sperm

Institute of Biology and Immunology of Reproduction-BAS, bul. Tsarigradsko Shose 73, p.c. 1113, Sofia, Bulgaria

Institute of Biology and Immunology of Reproduction- BAS, bul. Tsarigradsko shose 73, p.c. 1113, Sofi a, Bulgaria

Agricultural Institute, BG – Turgovishte, Bulgaria

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The aim of this study was to test the effect of extenders containing different sugar in their composition on ram sperm motility and pregnancy rate of ewe’s...

Effect of Different Extenders and Storage Periods on Motility and Fertility of Ram Sperm

Published Online: Nov 18, 2015

Page range: 85 - 89

Received: Jul 05, 2014

Accepted: Dec 01, 2014

DOI: https://doi.org/10.14432/j.macvetrev.2014.12.036

Keywords
ram, sperm extenders, motility, fertility

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Effect of Different Extenders and Storage Periods on Motility and Fertility of Ram Sperm

Published Online: Nov 18, 2015

Page range: 85 - 89

Received: Jul 05, 2014

Accepted: Dec 01, 2014

DOI: https://doi.org/10.14432/j.macvetrev.2014.12.036

Keywordsram, sperm extenders, motility, fertility

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
ram, sperm extenders, motility, fertility