<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Selenium is an essential micro-element in animal diet due to its high antioxidative properties. As a part of selenocystein it is an important constituent of the glutathione peroxidase (GPx) enzyme, which has a big importance for cell protection from oxidative damage. The aim of the present work was the investigation of the selenopyran effect on the antioxidative state of the pig ovary. The experiment was conducted with 18 gilts of Danube white breed randomly divided into two groups between 120 - 228 days of age. The animals received equal basal diets without selenium additives. The experimental gilts were injected once per month intramuscularly with oil solution of preparation selenopyran (9-phenylsymmetrical octahydroselenoxanthene) ensured 0.1 mgSe/kg live weight. After slaughtering, the ovaries were used for histological analysis and estimation of the selenium content in ovarian tissue by the atomic absorption spectroscopy method. The GPx activity in ovary homogenates using the colorimetric assay kit (BioVision) was measured. The expression of γ-glutamyl transpeptidase (GGT) in ovaries by immunochistochemical method was estimated. The selenopyran treatment leads to significant (P&lt;0.05) increase of the selenium level in blood and non-significant (P>0.05) in ovarian tissue. Enhancement of GPx activity in the ovaries of experimental group was observed (142.61±6.6 versus 122.28±3.4U/gP, P&lt;0.05). The GGT expression in the ovarian cortex cells, follicular fluid and in the erythrocytes of ovarian blood vessels in treated gilts was an evidence of active transport of glutathione from blood to the ovary tissue. The selenopyran treatment promotes the increase of the GPx dependent antioxidative defense in ovary of growing gilts.</p></abstract>

Selenium is an essential micro-element in animal diet due to its high antioxidative properties. As a part of selenocystein it is an important constituent of the glutathione peroxidase (GPx) enzyme, which has a big importance for cell protection from oxidative damage. The aim of the present work was the investigation of the selenopyran effect on the antioxidative state of the pig ovary. The experiment was conducted with 18 gilts of Danube white breed randomly divided into two groups between 120 - 228 days of age. The animals received equal basal diets without selenium additives. The experimental gilts were injected once per month intramuscularly with oil solution of preparation selenopyran (9-phenylsymmetrical octahydroselenoxanthene) ensured 0.1 mgSe/kg live weight. After slaughtering, the ovaries were used for histological analysis and estimation of the selenium content in ovarian tissue by the atomic absorption spectroscopy method. The GPx activity in ovary homogenates using the colorimetric assay kit (BioVision) was measured. The expression of γ-glutamyl transpeptidase (GGT) in ovaries by immunochistochemical method was estimated. The selenopyran treatment leads to significant (P<0.05) increase of the selenium level in blood and non-significant (P>0.05) in ovarian tissue. Enhancement of GPx activity in the ovaries of experimental group was observed (142.61±6.6 versus 122.28±3.4U/gP, P<0.05). The GGT expression in the ovarian cortex cells, follicular fluid and in the erythrocytes of ovarian blood vessels in treated gilts was an evidence of active transport of glutathione from blood to the ovary tissue. The selenopyran treatment promotes the increase of the GPx dependent antioxidative defense in ovary of growing gilts.

Improvement of the Antioxidative Status of Pig Ovaries by Selenopyran Treatment

Institute of Biology and Immunology of reproduction-BAS, bul. Tsarigradsko shose 73, p.c. 1113, Sofia, Bulgaria

Institute of Biology and Immunology of reproduction- BAS, bul. Tsarigradsko shose 73, 1113, Sofia, Bulgaria

Agricultural Institute , BG – 9700 Shumen, Bulgaria

Penza State Agricultural Academy, RU - 2771 Penza, Russia

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Improvement of the Antioxidative Status of Pig Ovaries by Selenopyran Treatment"}

Selenium is an essential micro-element in animal diet due to its high antioxidative properties. As a part of selenocystein it is an important constituent of the glutathione peroxidase (GPx) enzyme, which has a big importance for cell protection from oxidative damage. The aim of the present work was the investigation of the selenopyran effect on the antioxidative state of the pig ovary. The experiment was conducted with 18 gilts of Danube white breed randomly divided into two groups between 120 - 228 days of age. The animals received equal basal diets without selenium additives. The experimental gilts were injected once per month intramuscularly with oil solution of preparation selenopyran (9-phenylsymmetrical octahydroselenoxanthene) ensured 0.1 mgSe/kg live weight. After slaughtering, the ovaries were used for histological analysis and estimation of the selenium content in ovarian tissue by the atomic absorption spectroscopy method. The GPx activity in ovary homogenates using the colorimetric assay kit (BioVision) was measured. The expression of γ-glutamyl transpeptidase (GGT) in ovaries by immunochistochemical method was estimated. The selenopyran treatment leads to significant (P0.05) in ovarian tissue. Enhancement of GPx activity in the ovaries of experimental group was observed (142.61±6.6 versus 122.28±3.4U/gP, P<0.05). The GGT expression in the ovarian cortex cells, follicular fluid and in the erythrocytes of ovarian blood vessels in treated gilts was an evidence of active transport of glutathione from blood to the ovary tissue. The selenopyran treatment promotes the increase of the GPx dependent antioxidative defense in ovary of growing gilts.

Selenium is an essential micro-element in animal diet due to its high antioxidative properties. As a part of selenocystein it is an important constituent of...

Improvement of the Antioxidative Status of Pig Ovaries by Selenopyran Treatment

Published Online: Nov 18, 2015

Page range: 165 - 170

Received: Jun 02, 2014

Accepted: Aug 10, 2014

DOI: https://doi.org/10.14432/j.macvetrev.2014.08.021

Keywords
pig ovary, selenopyran, glutathione peroxidase (GPx)

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Improvement of the Antioxidative Status of Pig Ovaries by Selenopyran Treatment

Published Online: Nov 18, 2015

Page range: 165 - 170

Received: Jun 02, 2014

Accepted: Aug 10, 2014

DOI: https://doi.org/10.14432/j.macvetrev.2014.08.021

Keywordspig ovary, selenopyran, glutathione peroxidase (GPx)

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
pig ovary, selenopyran, glutathione peroxidase (GPx)