<abstract xmlns="http://www.w3.org/1999/xhtml">The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH•) bleaching assay kinetic profile. The kinetic profile of scavenging DPPH• for PE exhibited one kinetic period characterized by one kinetic constant. The second-order rate constant (<italic>k</italic>2) for the oxidation of PE by DPPH•, determined for the first time in this study, was 0.17 dm3g−1s−1. The obtained k2 value was compared to that of synthetic antioxidants and natural extracts used in the food industry. Kinetic analysis of PE antiradical effectiveness showed that the <italic>k</italic>2 was within the range values for natural colorants of fruit extracts and should be considered as a fast acting natural antioxidant source. The <italic>k</italic>2 parameter indicates the extent of oxidation inhibition that is based on all of the kinetic profiles of DPPH bleaching rather than single point measurements. For this reason, the kinetic analysis should become a necessary step for more precise antioxidative characterization of propolis.</abstract>

The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH•) bleaching assay kinetic profile. The kinetic profile of scavenging DPPH• for PE exhibited one kinetic period characterized by one kinetic constant. The second-order rate constant (k2) for the oxidation of PE by DPPH•, determined for the first time in this study, was 0.17 dm3g−1s−1. The obtained k2 value was compared to that of synthetic antioxidants and natural extracts used in the food industry. Kinetic analysis of PE antiradical effectiveness showed that the k2 was within the range values for natural colorants of fruit extracts and should be considered as a fast acting natural antioxidant source. The k2 parameter indicates the extent of oxidation inhibition that is based on all of the kinetic profiles of DPPH bleaching rather than single point measurements. For this reason, the kinetic analysis should become a necessary step for more precise antioxidative characterization of propolis.

Kinetic Profile of the Antioxidant Activity of Propolis Extract: 2,2-Diphenyl-1-Picrylhydrazyl Radical Bleaching Assay

Institute of Microbiology and Immunology, Faculty of Medicine

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH•)...

Kinetic Profile of the Antioxidant Activity of Propolis Extract: 2,2-Diphenyl-1-Picrylhydrazyl Radical Bleaching Assay

Published Online: Jun 21, 2018

Page range: 39 - 46

Received: Aug 21, 2017

Accepted: Dec 01, 2017

DOI: https://doi.org/10.2478/jas-2018-0004

Keywords
antioxidative activity, kinetic analysis, proplis extract, second order rate constant

© 2018 Snježana Mirković et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Kinetic Profile of the Antioxidant Activity of Propolis Extract: 2,2-Diphenyl-1-Picrylhydrazyl Radical Bleaching Assay

Published Online: Jun 21, 2018

Page range: 39 - 46

Received: Aug 21, 2017

Accepted: Dec 01, 2017

DOI: https://doi.org/10.2478/jas-2018-0004

Keywordsantioxidative activity, kinetic analysis, proplis extract, second order rate constant

© 2018 Snježana Mirković et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
antioxidative activity, kinetic analysis, proplis extract, second order rate constant