<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Echinococosis is a zoonotic disease caused by the larval stages of <italic>Echinococcus</italic> spp. that occurs in most parts of the world. Herein, we aimed to evaluate the genotypes of isolated hydatid cysts from slaughtered animals in Shush county, southwestern Iran. Totally, 96 hydatid cysts were collected, including 11 buffaloes, 13 cattle, 12 goat and 60 sheep. The PCR was done by a primer pair (BDI and 4s) to amplify ITS1 fragment. Four restriction endonucleases including AluI, HpaII, RsaI, and TaqI were used for RFLP products and enzymatic reactions were electrophoresed. Finally, twenty PCR products were sent for sequencing and phylogenetic tree was drawn with MEGA6. Molecular identification of 96 hydatid cysts demonstrated a distinctive 1000 bp fragment in all samples from four animal hosts. RFLP analysis showed similar digestion patterns in all samples. AluI digestion yielded 800 bp and 200 bp fragments, HpaII digestion made 700 bp and 300 bp fragments and RsaI digestion entailed 655 and 345segments. Moreover, TaqI rendered no digestion pattern on rDNA-ITS1 region. Additionally, <italic>E. granulosus</italic> sensu stricto (G1-3 complex) was the prevailing genotype in all livestock samples, according to PCR-RFLP and sequencing analyses.</p></abstract>

Echinococosis is a zoonotic disease caused by the larval stages of Echinococcus spp. that occurs in most parts of the world. Herein, we aimed to evaluate the genotypes of isolated hydatid cysts from slaughtered animals in Shush county, southwestern Iran. Totally, 96 hydatid cysts were collected, including 11 buffaloes, 13 cattle, 12 goat and 60 sheep. The PCR was done by a primer pair (BDI and 4s) to amplify ITS1 fragment. Four restriction endonucleases including AluI, HpaII, RsaI, and TaqI were used for RFLP products and enzymatic reactions were electrophoresed. Finally, twenty PCR products were sent for sequencing and phylogenetic tree was drawn with MEGA6. Molecular identification of 96 hydatid cysts demonstrated a distinctive 1000 bp fragment in all samples from four animal hosts. RFLP analysis showed similar digestion patterns in all samples. AluI digestion yielded 800 bp and 200 bp fragments, HpaII digestion made 700 bp and 300 bp fragments and RsaI digestion entailed 655 and 345segments. Moreover, TaqI rendered no digestion pattern on rDNA-ITS1 region. Additionally, E. granulosus sensu stricto (G1-3 complex) was the prevailing genotype in all livestock samples, according to PCR-RFLP and sequencing analyses.

Determination of <italic>Echinococcus granulosus</italic> genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP

Ahvaz Jundishapur University of Medical Sciences

Department of Parasitology, School of Medicine

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Determination of  genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP

Echinococosis is a zoonotic disease caused by the larval stages of Echinococcus spp. that occurs in most parts of the world. Herein, we aimed to evaluate the...

Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP

Published Online: Jul 27, 2019

Page range: 196 - 201

Received: Jan 10, 2019

Accepted: Jun 14, 2019

DOI: https://doi.org/10.2478/helm-2019-0023

Keywords
genotypes, livestock, Shush County, PCR-RFLP

© 2019 S. Fallahizadeh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP

Published Online: Jul 27, 2019

Page range: 196 - 201

Received: Jan 10, 2019

Accepted: Jun 14, 2019

DOI: https://doi.org/10.2478/helm-2019-0023

Keywordsgenotypes, livestock, Shush County, PCR-RFLP

© 2019 S. Fallahizadeh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
genotypes, livestock, Shush County, PCR-RFLP