<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Six simple sequence repeats (SSR) markers were developed from expressed sequence tags (ESTs) in the genus <italic>Larix</italic>. Based on evaluation with 49 <italic>L. kaempferi</italic> genotypes, the number of alleles per locus ranged from two to four, and the expected (<italic>He</italic>) and observed (<italic>Ho</italic>) heterozygosity values were 0.225−0.694 and 0.201−0.656, respectively. The inbreeding coffcient (<italic>F</italic><sub>IS</sub>) for all loci were less than zero except that LAReSSR85 was 0.4383. All the six EST-SSR markers were transferable to <italic>L. gmelini</italic>, <italic>L. olgensis</italic> var <italic>Koreana</italic>, <italic>L. principisrupprechtii</italic> and <italic>L. olgensis</italic>. BlastX analysis showed that five of the EST-SSRs were homologous to known genes. The six EST-SSR markers developed here can be valuable for biological applications in <italic>Larix</italic>.</p></abstract>

Six simple sequence repeats (SSR) markers were developed from expressed sequence tags (ESTs) in the genus Larix. Based on evaluation with 49 L. kaempferi genotypes, the number of alleles per locus ranged from two to four, and the expected (He) and observed (Ho) heterozygosity values were 0.225−0.694 and 0.201−0.656, respectively. The inbreeding coffcient (FIS) for all loci were less than zero except that LAReSSR85 was 0.4383. All the six EST-SSR markers were transferable to L. gmelini, L. olgensis var Koreana, L. principisrupprechtii and L. olgensis. BlastX analysis showed that five of the EST-SSRs were homologous to known genes. The six EST-SSR markers developed here can be valuable for biological applications in Larix.

Short note: Development of Six EST-SSR Markers in Larch

Research Institute of Forestry, Chinese Academy of Forestry

Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Short note: Development of Six EST-SSR Markers in Larch"}

Six simple sequence repeats (SSR) markers were developed from expressed sequence tags (ESTs) in the genus Larix. Based on evaluation with 49 L. kaempferi...

Short note: Development of Six EST-SSR Markers in Larch

Published Online: Aug 05, 2017

Page range: 161 - 163

Received: Jan 21, 2011

DOI: https://doi.org/10.1515/sg-2011-0022

Keywords
Expressed sequence tag (EST), Simple sequence repeats (SSRs), cross-species transferability

© 2011 X. Yang et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Short note: Development of Six EST-SSR Markers in Larch

Published Online: Aug 05, 2017

Page range: 161 - 163

Received: Jan 21, 2011

DOI: https://doi.org/10.1515/sg-2011-0022

KeywordsExpressed sequence tag (EST), Simple sequence repeats (SSRs), cross-species transferability

© 2011 X. Yang et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Expressed sequence tag (EST), Simple sequence repeats (SSRs), cross-species transferability