<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The purpose of this work was to identify GHRH, GH and PRL gene polymorphisms in Anatolian water buffalo by means of the PCR-RFLP method. A total of 126 buffalo were included in this study. PCR amplification gave a 451 bp band for the GHRH gene, a 221 bp band for the GH gene and a 156 bp band for the PRL gene. The PCR products were digested by <italic>Hae</italic>III for the GHRH gene, <italic>Alu</italic>I for the GH gene and <italic>Rsa</italic>I for the PRL gene. The GH/<italic>Alu</italic>I and PRL/<italic>Rsa</italic>I polymorphisms were found to be polymorphic, while the GHRH/<italic>Hae</italic>III polymorphism was not found in Anatolian water buffalo. The frequencies of GH-L (0.87) and PRL-A (0.55) alleles were found to be high in the examined Anatolian water buffalo. The chi-square test showed that the Anatolian water buffalo were in Hardy-Weinberg (HW) equilibrium for the GH gene while significant deviation was observed from HW equilibrium for the PRL gene. The present study is the first to examine GHRH/<italic>Hae</italic>III, GH/<italic>Alu</italic>I and PRL/<italic>Rsa</italic>I polymorphisms in Anatolian water buffalo.</p></abstract>

The purpose of this work was to identify GHRH, GH and PRL gene polymorphisms in Anatolian water buffalo by means of the PCR-RFLP method. A total of 126 buffalo were included in this study. PCR amplification gave a 451 bp band for the GHRH gene, a 221 bp band for the GH gene and a 156 bp band for the PRL gene. The PCR products were digested by HaeIII for the GHRH gene, AluI for the GH gene and RsaI for the PRL gene. The GH/AluI and PRL/RsaI polymorphisms were found to be polymorphic, while the GHRH/HaeIII polymorphism was not found in Anatolian water buffalo. The frequencies of GH-L (0.87) and PRL-A (0.55) alleles were found to be high in the examined Anatolian water buffalo. The chi-square test showed that the Anatolian water buffalo were in Hardy-Weinberg (HW) equilibrium for the GH gene while significant deviation was observed from HW equilibrium for the PRL gene. The present study is the first to examine GHRH/HaeIII, GH/AluI and PRL/RsaI polymorphisms in Anatolian water buffalo.

Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo

Department of Veterinary Animal Science, Graduate School of Health Sciences

Department of Genetics, Faculty of Veterinary Medicine

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The purpose of this work was to identify GHRH, GH and PRL gene polymorphisms in Anatolian water buffalo by means of the PCR-RFLP method. A total of 126 buffalo...

Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo

Published Online: Oct 27, 2017

Page range: 1053 - 1062

Received: Aug 08, 2016

Accepted: Mar 08, 2017

DOI: https://doi.org/10.1515/aoas-2016-0100

Keywords
Anatolian water buffalo, growth hormone, growth hormone releasing hormone, prolactin hormone, RFLP

© 2017 Mehmet Akif Konca et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo

Published Online: Oct 27, 2017

Page range: 1053 - 1062

Received: Aug 08, 2016

Accepted: Mar 08, 2017

DOI: https://doi.org/10.1515/aoas-2016-0100

KeywordsAnatolian water buffalo, growth hormone, growth hormone releasing hormone, prolactin hormone, RFLP

© 2017 Mehmet Akif Konca et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Anatolian water buffalo, growth hormone, growth hormone releasing hormone, prolactin hormone, RFLP