<abstract xmlns="http://www.w3.org/1999/xhtml">The β-2-adrenergic receptor (<italic>ADRB2</italic>) gene has an important impact on smooth muscle relaxation, including the smooth muscles of the uterus. The results of previously published studies of the association between the <italic>ADRB2</italic> rs1042713 polymorphism and spontaneous preterm birth (SPTB) were inconsistent. We evaluated the association between <italic>ADRB2</italic> and SPTB in a case-control association study in a Slovenian sample population and performed a meta analysis of previously published studies. No association was found between the polymorphism in the <italic>ADRB2</italic> gene and SPTB in the Slovenian sample of 98 SPTB patients and 135 controls under dominant [χ2 = 0.01, <italic>p</italic> = 0.92, odds ratio (OR) = 1.03, 95% confidence interval (95% CI) = 0.52-2.04), recessive (χ2 = 0.01, <italic>p</italic> = 0.92, OR = 0.98, 95% CI = 0.57-1.70) and codominant genetic models (χ2 = 0.01, <italic>p</italic> = 0.92, OR = 0.99, 95% CI = 0.59-1.68). The meta analysis of a pooled sample of 404 SPTB patients and 878 controls suggested no association of <italic>ADRB2</italic> polymorphism and SPTB under dominant (OR = 1.12, 95% CI = 0.81-1.54) and recessive genetic models (OR = 0.84, 95% CI = 0.64-1.12). These findings suggest no association between the <italic>ADRB2</italic> rs1042713 gene polymorphism and SPTB. Further association studies with larger sample sizes are needed.</abstract>

The β-2-adrenergic receptor (ADRB2) gene has an important impact on smooth muscle relaxation, including the smooth muscles of the uterus. The results of previously published studies of the association between the ADRB2 rs1042713 polymorphism and spontaneous preterm birth (SPTB) were inconsistent. We evaluated the association between ADRB2 and SPTB in a case-control association study in a Slovenian sample population and performed a meta analysis of previously published studies. No association was found between the polymorphism in the ADRB2 gene and SPTB in the Slovenian sample of 98 SPTB patients and 135 controls under dominant [χ2 = 0.01, p = 0.92, odds ratio (OR) = 1.03, 95% confidence interval (95% CI) = 0.52-2.04), recessive (χ2 = 0.01, p = 0.92, OR = 0.98, 95% CI = 0.57-1.70) and codominant genetic models (χ2 = 0.01, p = 0.92, OR = 0.99, 95% CI = 0.59-1.68). The meta analysis of a pooled sample of 404 SPTB patients and 878 controls suggested no association of ADRB2 polymorphism and SPTB under dominant (OR = 1.12, 95% CI = 0.81-1.54) and recessive genetic models (OR = 0.84, 95% CI = 0.64-1.12). These findings suggest no association between the ADRB2 rs1042713 gene polymorphism and SPTB. Further association studies with larger sample sizes are needed.

Polymorphism of the <italic>ADRB2</italic> rs1042713 gene is not associated with spontaneous preterm birth: Analyses in a Slovenian sample and meta analysis

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Polymorphism of the  rs1042713 gene is not associated with spontaneous preterm birth: Analyses in a Slovenian sample and meta analysis

The β-2-adrenergic receptor (ADRB2) gene has an important impact on smooth muscle relaxation, including the smooth muscles of the uterus. The results of...

Genetic Model			W_SPTBvs.W_C
		OR (95% CI)	p Value	χ²
Dominant	GA+GG vs. AA	1.03 (0.52-2.04)	0.92	0.01
Recessive	AA+GA vs. GG	0.98 (0.57-1.70)	0.92	0.01
Codominant	AA+GG vs. GA	0.99 (0.59-1.68)	0.92	0.01

Parameters	SPTB	Controls	p Value
Mean maternal age (years)	30.20 ± 4.59	30.10 ± 3.92	0.28
Smoking during pregnancy % (n)	8.3 (8)	2.9 (4)	0.15
Previous SPTB % (n)	12.4 (12)	4.3 (6)	0.08
In vitro fertilization % (n)	4.1 (4)	1.4 (2)	0.31
Urogenital infections % (n)	7.2 (7)	4.3 (6)	0.35
High blood pressure % (n)	7.2 (7)	4.3 (6)	0.35
Stressful events during pregnancy % (n)	3.1 (3)	4.3 (6)	0.50

Refs.	Year	Country	Population	PPROM Excluded	SPTB Cases Criteria	Case Sample Size	Control Criteria	Control Group Size
this study	2017	Slovenia	Caucasian	no	GA <37	98	GA >37	135
[30]	2013	Korea	Asian	no	GA <37	166	GA >37	289
[31]	2004	Hungary	Caucasian	no	GA <37	32	GA >37	127
[33]	2002	Turkey	Caucasian	yes	GA <37	80	GA >37	76
[32]	2002	USA	Hispanic	no	GA <37	28	GA >37	251

Parameters	Genotype Frequencies n (%)					Allele Frequencies (n) %
	AA	GA	GG	p Value	χ²	A	G	p Value	χ²
SPTB cases	17 (17.3)	48 (49.0)	33 (33.7)	0.99	0.01	65 (42.0)	81 (42.0)	1.0	0
Controls	24 (18.0)	66 (49.0)	45 (33.0)			90 (58.0)	111 (58.0)

Polymorphism of the ADRB2 rs1042713 gene is not associated with spontaneous preterm birth: Analyses in a Slovenian sample and meta analysis

Article Category: Original Article

Published Online: Dec 29, 2017

Page range: 35 - 41

DOI: https://doi.org/10.1515/bjmg-2017-0032

Keywords
β-2-Adrenergic receptor () gene, Case-control association study, Meta analysis, Risk factor, Spontaneous preterm birth (SPTB)

© 2017 Peterlin A, Maver A, Jan Z, Lovrecic L, Tul N, Peterlin B, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Association of ADRB2 rs1042713 with SPTB under different genetic models.

Characteristics of 98 mothers with SPTB and 135 controls.

Characteristics of studies included in the meta analysis.

Genotype and allelic distribution of the ADRB2 rs1042713 polymorphism of the 98 SPTB patients and 135 controls.

Polymorphism of the ADRB2 rs1042713 gene is not associated with spontaneous preterm birth: Analyses in a Slovenian sample and meta analysis

Article Category: Original Article

Published Online: Dec 29, 2017

Page range: 35 - 41

DOI: https://doi.org/10.1515/bjmg-2017-0032

Keywordsβ-2-Adrenergic receptor () gene, Case-control association study, Meta analysis, Risk factor, Spontaneous preterm birth (SPTB)

© 2017 Peterlin A, Maver A, Jan Z, Lovrecic L, Tul N, Peterlin B, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Association of ADRB2 rs1042713 with SPTB under different genetic models.

Characteristics of 98 mothers with SPTB and 135 controls.

Characteristics of studies included in the meta analysis.

Genotype and allelic distribution of the ADRB2 rs1042713 polymorphism of the 98 SPTB patients and 135 controls.

Keywords
β-2-Adrenergic receptor () gene, Case-control association study, Meta analysis, Risk factor, Spontaneous preterm birth (SPTB)