Cytology of the Pituitary Gonadotrophs, Histological Characteristics of Interrenal and Chromaffin Cells in Relation to Testicular Activities in Mystus Vittatus (Siluriformes, Bagridae) During Growth, Maturation and Spawning Phases

M. Banerjee 1 , S. Ghosh 1  and P. Chakrabarti 1
  • 1 Fisheries Laboratory, Department of Zoology, The University of Burdwan, 713104, Burdwan, India


The histological changes observed in the pituitary corticotrophs, gonadotrophs, adrenocortical tissues and testicular cells in M. vittatus (Bloch, 1794) have been studies during growth, maturation and spawning phases. The studies based on the changes observed in the cell types, shape and size of the cells of the adrenocortical tissues, testes and the overall percentage of gonadotroph (GTH) and thyrotroph (TSH) cells of the pituitary. However, during growth phase, in proximal pars distalis (PPD) the considerable increment of GTH and TSH have been observed having intense aniline blue stain. The corticotrophs (ACTH) also showed significant accumulation of fuchsinophilic cytoplasmic granules. The cytoplasmic features and the architecture of the interrenal cells were well coincident with the increase of different spermatogenic cells. During the maturation phase dense granulation in the GTH and TSH cells appeared to be concomitant with the spermiation. The amount of cytoplasmic granules of the interrenal cells increased than chromaffin cells and was well coincidence with the increase of spermatids and spermatozoa. The hyperactive and vacuolated features of the interrenal cells during spawning phase appeared to be concomitant with the final process of spermiation.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Ahsan, S. N., 1966. Effects of gonadotropic hormones on male hypophysectomized lake chub, Couesius plumbeus. Can. J. Zool., 44 (4), 703–717.

  • Assem, S. S., El-Boray K. F. 2001. Cell type distribution and comparative studies of gonadotropin cells in the pituitary gland of female Rhabdosargus haffara reared in saline and brakish water. Egypt. J. Aqua. Res., 27, 109–134.

  • Ball, J. N., Baker, B. I. 1969. The pituitary gland: Anatomy and Histophysiology. In: Hoar, M. S., Randall, D. J. eds. Fish Physiology. Academic Press, New York, 11, 1–110.

  • Borella, M. I., Morris, C. R., Gazola, R., Rossana, V., Bernardino, G. 1999. Pituitary gland, gonads and interrenal cells gland of the immature pacu Piaractus mesopotamicus (Teleost, Characidae): morphological study. B. Tec. Cepta, Pirassununga, 12, 57–70.

  • Chakrabarti, P. 2014. Histological features of interrenal and chromaffin cells in relation to testicular activities in Notopterus notopterus (Pallas). Int. J. Fish. Aqua Stud., 1 (3), 206–213.

  • Civinini, A, Padula, D, Gallo, V. P., 2001. Ultrastructure and histochemical study on the interrenal cells of the male stickleback (Gasterosteus aculeatus), in relation to the reproductive annual cycle. J. Anat., 199, 303–316.

  • Delahunty, G., deVlaming, V. L., 1980. Seasonal relationship of ovary weight, liver weight and fat stores with body weight in the gold fish, Carassius auratus. J. Fish. Biol., 16, 5–13.

  • El-Sakhaway, M. A., El-Shamma, M. A., Abd Rabou, M. I., El-Seba, A. A., Hussein, S. H., 2011. Seasonal histology and histochemistry of the adenohypophysis of Nile Tilapia (Oreochromis niloticus). J. Vet. Anat., 4, 39–60.

  • El-Zoghby, I. M. A., Bakry, H. H., Ghallab, A. M., Emam, M. A., 2008. Histological studies on the pituitary gland of catfish (Clarias lazera) during different seasons. The 32nd Sc. Conf. Egypt. Soc. Hist. Cyto., 24–35.

  • Gadekar, G. P. 2014. Studies on the seasonal histomorphological changes in the ovary of Indian major carp, Labeo rohuta (Ham.). The Bioscan, 9 (3), 1037–1042.

  • Ganguli, S., Chakrabari, P. 1995. Annual cyclical rhythm is the cytology of the testis of the freshwater featherback, Notopterus notopterus (Pallas). J. Freshwater Biol., 7, 187–193.

  • Gazola, R., Borella, M. I., Val-Sella, M. V., Fava de Morases, F., Bernardino, G., 1995. Histophysiological aspects of the interrenal of the pacu female, Piaractus mesopotamicus (Teleostei, Cypriniformes). B. Tec. Cepta, Pirassununga, 8, 1–12.

  • Gomori, G. 1941. Observation with differential stains on human islets of Langerhans. Am. J. Path., 17, 395.

  • Green, J. D., Maxwell, D. S., 1959. Comparative anatomy of the hypophysis and observations on the mechanism of neurosecretion. Comparative endocrinology, 368–392.

  • Jones, I.C., Phillips, J.G. 1986. The adrenal and interrenal gland. In: Pang, P. K. T., Schreibman, M. P., eds. Vertebrate Endocrinology. Springer, New York, 319–350.

  • Joshi, B. N., Sathyanesan, A. G. 1980. A histochemical study on the adrenal components of the teleost Cirrhinus mrigala (Hamilton). Z. Mikrosk. Anat. Forsch., 94, 327–336.

  • Kharat, S. S., Khillare, Y. K. 2013. Morphology and histology of pituitary gland of torrential stream fish Nemacheilus mooreh (Sukes). Int. J. Bioassays., 2 (8), 1079–1084.

  • Krishnan, L., Diwan, A. D. 1990. Seasonal changes in gonads and their relationship with gonadotrophs of the pituitary in Etroplus suratensis (Bloch). Mar. Biol. Ass., India, 32 (1&2), 5–9.

  • Lethimonier, C., Madigou, T., Munoz-Cueto, J. A., Lareyre, J. J., Kah, O. 2004. Evolutionary aspects of GnRHs, GnRH neuronal system and GnRH receptors in teleost fish. Gen. Comp. Endocrinal., 135 (1), 1–16.

  • Mallory, F. B. 1936. The aniline blue collagen stain. Stain. Tech., 11, 101.

  • Mandal, S., Sinha, G. M. 1985. Adrenocortical cell types in an Indian freshwater major carp, Catla catla (Hamilton). Gegenbaurs Morphologische Jaharbuchen, 131, 81–92.

  • Mc Manus, J. F.A. 1948. Histological and histochemical users of periodic acid. Stain. Tech., 23 (3), 99–108.

  • Morandini, L., Honji, R. M., Ramallo, M. R., Moreira, R. G., Pandolfi, M. 2014. The interrenal gland in males of the cichilid fish Cichlasoma dimerus : relationship with stree and the establishment of social hierarchies. Gen. Comp. Endocrinal., 195, 88–98.

  • Mukhopadhyay, S., Sinha, G. M. 1986. Annual cyclical changes in the testicular activity of an Indian freshwater major carp Labeo rohita (Hamilton). Gegenbaurs Morphologische Jaharbuchen, 132 (2), 303–321.

  • Parhar, I. S., Soga, T., Ogawa, S., Sakuma, Y. 2003. FSH and LH-b subunits in the preoptic nucleus: ontogenic expression in teleost. Gen. Comp. Endocrinal., 132, 369–378.

  • Pickford, G. E., Atz, J. W. 1957. The physiology of the pituitary gland of fishes. New York Zoological Society, New York, 613.

  • Rai, B. P. 1966. The hypophysis of the mahseer, Tor (Barbus) tor (Ham.). Anat. Anz., 120 (4), 360–374.

  • Reid, S. G., Bernier, N. J., Perry, S. F. 1998. The adrenergic stress response in fish: Control of catecholamine storage and release. Comp. Biochem. Physiol., C120, 1–27.

  • Sampour, M. 2008. The study of adrenal chromaffin of fish, Carassius auratus (Teleostei). Pak. J. Biol. Sci., 11, 1032–1036.

  • Sathyanesan, A. G. 1960. Correlative cyclical changes in the pituitary and gonads of Mystus seenghala (Sykes) and Barbus stigma (Cuv. and Val.). J. Zoological Soc., India, 12 (2), 175–190.

  • Ursani, T. J., Shaikh, S. A., Soomro, S. M., Malik, S. 2012. Histological changes in piyuitary gonadotrophic hormone cells in relation to seasonal changes in ovaries of the fish Oreochromis mossambicus. Sindh Univ. Res. J. Sci. Ser., 44 (1), 107–114.

  • Wen, J., Xie, J., Gui, J. 2003. cDNA cloning and characterization of a novel SNX gene differentially expressed in previtellogenic oocytes of gibel carp. Comp. Biochem. Physiol., 136, 451–461.

  • Yadav, B. N., Singh, B. R., Munsi, J. S. D. 1970. Histophysiology of the adrenocortical tissue of an air-breathing fish, Heteropneustes fossilis (Bloch). Mikroskopie, 26, 41–49.


Journal + Issues