Blood samples of “yellow” wagtails collected in the areas geographically representing the Middle Volga breeding populations of these species were investigated. After isolation of mtDNA barkoding of studied “yellow” wagtails species was conducted. Amplification of the subunit of cytochrome oxidase I gene used as a genetic marker for the comparison of the samples was carried out. After sequencing and sequence alignment of gene cytochrome c-oxidase I, based on the comparison of genetic distances between individuals of the studied species using Jalview phylogenetic trees of populations of species Motacilla flava Linnaeus, 1758 and Motacilla citreola Pallas, 1776 were constructed.
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Jalview - URL: http://www.jalview.org
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