Tomato Carrying MI-1.2 Gene as a Host-Plant to the Two-Spotted Spider Mite (Tetranychus urticae Koch): Results of Laboratory Evaluation

Open access

Tomato Carrying MI-1.2 Gene as a Host-Plant to the Two-Spotted Spider Mite (Tetranychus urticae Koch): Results of Laboratory Evaluation

Mi-1.2 gene contributes to the resistance of tomato plants against some species of herbivores (i.e. nematodes, aphids) that are sap-feeders. In this study, two genotypes of tomato (Solanum lycopersicum L. syn. Lycopersicon esculentum Mill.) plants with dominant (Motelle cv) and recessive (Moneymaker cv) Mi-1.2 alleles were evaluated as a host-plant to the two-spotted spider mite (Tetranychus urticae Koch, Acari: Tetranychidae). Plants were grown under glasshouse conditions. The effect of tomato genotypes on T. urticae bionomy was estimated on cut leaflets in controlled photoperiod, temperature and humidity (L/D=16/8 h; D/N temp.=23/20°C; RH=70%). Results of laboratory evaluation showed that both the bionomy and population parameters of T. urticae reached a very similar or even higher value on plants with dominant Mi-1.2 allele than on plants with recessive one. A relatively high value of T. urticae parameters on plants resistant to root knot nematodes indicates that the mite has potential to develop rapidly. This allows the mite population to reach the density causing marked damage to plant in a short time. It is concluded that the presence of Mi-1.2 gene product in tomato plants does not significantly affect the reproductive potential of T. urticae. Therefore, Mi-1.2 gene cannot be a satisfying source of plant resistance to this mite-herbivore generalist.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Andrewartha H. G. Birch L. C. 1954. The distribution and abundance of animals. University of Chicago Press Chicago USA.

  • Carey J. S. Bradley J. W. 1982. Developmental rates vital schedules sex ratios and life tables for T. urticae T. turkestani and T. pacificus (Acarina: Tetranychidae). Acarologia XXIII (4): 333-345.

  • Casteel C. L. Walling L. L. Paine T. D. 2006. Behavior and biology of the tomato psyllid Bactericerca cockerelli in response to the Mi-1.2 gene. Entomol. Exp. Appl. 121: 67-72. [DOI: 10.1111/j.1570-8703.2006.00458.x].

  • Czajkowska B. Kiełkiewicz M. Puchalska E. 2003. Population parameters as an indicator of susceptibility of ornamental coniferous plants to spruce spider mite. J. Plant Protection Res. 43 (2): 155-162.

  • Godzina M. Staniaszek M. Kiełkiewicz M. 2010. Relevance of the Mi23 marker and the potato aphid biology as indicators of tomato plant (Solanum lycopersicum L.) resistance to some pests. Veget. Crops Res. Bull. 72: 25-33.

  • Goggin F. L. Williamson V. M. Ullman D. E. 2001. Variability in the response of Macrosiphum euphorbiae and Myzus persicae (Hemiptera: Aphididae) to the tomato resistance gene Mi. Environ. Entomol. 30: 101-106. [DOI: 10.1603/0046-225X-30.1.101]

  • Goggin F. L. Gowri S. Williamson V. M. Ullman D. E. 2004. Developmental regulation of Mi-mediated aphid resistance is independent of Mi-1.2 transcript levels. Mol. Plant-Microbe Interact. 17: 532-536. [DOI:10.1094/MPMI.2004.17.5.532].

  • Goggin F. L. Jia L. Shah G. Hebert S. Williamson V. M. Ullman D. E. 2006. Heterologous expression of the Mi-1.2 gene from tomato confers resistance against nematodes but not aphids in eggplant. Mol. Plant-Microbe Interact. 19: 383-388. [DOI: 10.1094/MPMI-19-0383].

  • Hussey N. W. Scopes N. E. A. 1985. Biological pest control: The glasshouse experience (Hussey N. W. Scopes N. E. A ed). Cornell University Press New York USA.

  • Jiang Y. X. Nombela G. Muniz M. 2001. Analysis by DC - EPG of the resistance to Bemisia tabaci on Mi - tomato line. Entomol. Exp. Appl. 99: 295-302. [DOI: 10.1046/j.1570-7458.2001.00828.x].

  • Kaloshian I. Lange W. H. Williamson V. M. 1995. An aphid resistance locus is tightly linked to the nematode resistance gene Mi in tomato. Proc. Natl. Acad. Sci. USA 92: 622-625.

  • Kielkiewicz M. 1996 a. Hypersensitive response of tomato leaf tissues towards Tetranychus cinnabarinus Boisd. (Tetranychidae) feeding. pp: 47-49. In: Acarology IX. (R. Mitchell D. J. Horn G. R. Needham W. C. Welbourn ed.). Columbus Ohio USA.

  • Kielkiewicz M. 1996 b. Dispersal of Tetranychus cinnabarinus on various tomato cultivar. Entomol. Exp. Appl. 80: 254-257.

  • Kiełkiewicz M. 2003. Strategie obronne roślin pomidorów (Lycopersicon esculentum Miller) wobec przędziorka szklarniowca (Tetranychus cinnabarinus Boisduval Acari: Tetranychidae). Wydawnictwo SGGW. Warszawa. [in Polish]

  • Milligan S. B. Bodeau J. Yaghoobi J. Kaloshian I. Zabel P. Williamson V. M. 1998. The root - knot nematode resistance gene Mi from tomato is a member of leucine zipper nucleotide binding leucine - rich repeat family of plant genes. Plant Cell. 10: 1307-1319.

  • Nombela G. Beitia F. Muniz M. 2001. A differential interaction study of Bemisia tabaci Q - biotype on commercial tomato varieties with or without the Mi resistance gene and comparative host responses with the B - biotype. Entomol. Exp. Appl. 98: 339-344. [DOI: 10.1046/j.1570-7458.2001.00790.x].

  • Nombela G. Williamson V. M. Muniz M. 2003. The root - knot nematode resistance gene Mi-1.2 of tomato is responsible for resistance against the whitefly Bemisia tabaci. Mol. Plant-Microbe Interact. 16: 645-649. [DOI:10.1094/MPMI.2003.16.7.645].

  • Rossi M. Goggin F. L. Milligan S. B. Kaloshian I. Ullman D. E. Williamson V. M. 1998. The nematode resistance gene Mi of tomato confers resistance against the potato aphid. Proc. Natl. Acad. Sci. USA 95: 9750-9754.

  • Sabelis M. W. 1991. Life history evolution of spider mites. pp. 23-49. In: The Acari - Reproduction Development and Life-history Strategies. (Schuster R. & Murphy P. W. ed.). Chapman and Hall London United Kingdom.

  • Smith C. M. 2005. Constitutive and induced resistance genes. pp. 423. In: Plant resistance to arthropods. Molecular and Conventional Approaches (Smith C. M. ed.). Springer. Dordrecht. The Netherlands.

  • Witul A. 1992. Life history parameters of Tetranychus cinnabarinus on glass-house plants. EPPO Bulletin. 22: 521-528.

  • Witul A. Kielkiewicz M. 1996. How young gerbera affects bionomy of Tetranychus cinnabarinus Boisd. (Acarina: Tetranychidae). pp. 33-35. In: Acarology IX. (R. Mitchell D. J. Horn G. R. Needham W. C. Welbourn ed.). Columbus Ohio USA.

  • Witul A. Kielkiewicz M. 1999. Life-history parameters of two closely related forms of the Tetranychus urticae - complex on different host plants. pp. 399-404. In: Ecology and Evolution of the Acari. (J. Bruin L. P. S. van der Geest M. W. Sabelis ed.). Kluver Academic Publishers Dordrecht/Boston/London. The Netherlands/USA/UK.

Search
Journal information
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 250 131 4
PDF Downloads 87 50 3