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At present, high traffic intensity level on the roads currently saturates a great demand for individual transportation. Mobility is an important part of everyday activities in cities. The increasing number of passenger and freight vehicles on the widening transport infrastructure raises several problems. The range of use of public passenger transport does satisfy enough the citizens needs and its use is constantly decreasing, especially in the new EU countries. One of the reasons is also the growing sale and production of new passenger cars in the EU, resulting in congestion in the road infrastructure of cities and environmental problems. These issues are dealt with the modern City Logistics concept. The contribution focused on the basic principles of city logistics, the reasons for its development and, in particular, the possibilities of practical use. An example is the city of Trenčín in Slovakia, where a regional rail passenger transport is proposed for city traffic service including Park and Ride system.