Effect of vitamin E (DL-all-rac-a-tocopherol acetate) and nano particles of selenium on growth, survival, body composition and whole body glutathione peroxidase (GPX) and malondialdehyde (MDA) in Rutilus kutum (Kamensky, 1901)

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The effect of vitamin E (100 mg kg−1) and nano-selenium (1 mg kg−1), which have a nutritional relationship separately and in combination, was investigated on growth, survival, carcass composition, body glutathione peroxidase activity, and body malondialdehyde content of Rutilus kutum. Results showed that vitamin E is capable of improving growth, FCR and WG in Kutum fingerlings; however, nano-selenium is not. According to this study, vitamin E can improve growth and selenium can improve glutathione peroxidase activity in Rutilus kutum larvae.

1. Abdolhay H. A., Daud S. K., Ghilkolahi S. R., Pourkazemi M., Siraj S. S. and Satar M. A., 2011 − Fingerling production and stock enhancement of Mahisefid (Rutilus frisii kutum) lessons for others in the south of Caspian Sea, Reviews in Fish Biology and Fisheries, 21, 247-257.

2. Afkhami M., Bastami K. D., Shabani N. and Soltani F., 2014 – A survey on ionic and metabolite factors of blood serum in Kutum (Rutilus frisii kutum), Transylvanian Review of Systematical and Ecological Research, 16.2, The Wetlands Diversity, 127-132.

3. Amlashi A. S., Falahatkar B., Sattari M. and Gilani M., 2011 − Effect of dietary vitamin E on growth, muscle composition, hematological and immunological parameters of sub-yearling beluga (Huso huso) L., Fish and shellfish immunology, 30, 807-814.

4. AOAC, 2002 − Official Methods of Analysis of AOAC International (17th edition), Arlington, VA.

5. Bell J., Cowey C., Adron J. and Pirie B., 1987 − Some effects of selenium deficiency on enzyme activities and indices of tissue peroxidation in Atlantic salmon parr (Salmo salar), Aquaculture, 65, 43-54.

6. Bell J., Cowey C., Adron J. and Shanks A. M., 1985 − Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout (Salmo gairdneri), British Journal of Nutrition, 53, 149-157.

7. Bell J., Pirie B., Adron J. and Cowey C., 1986 − Some effects of selenium deficiency on glutathione peroxidase (EC 1.11. 1.9) activity and tissue pathology in rainbow trout (Salmo gairdneri), British Journal of Nutrition, 55, 305-311.

8. Chow C. K., 1991 − Vitamin E and oxidative stress, Free Radical Biology and Medicine, 11, 215-232.

9. Ebrahimi G., Ouraji H., Firouzbakhsh F. and Makhdomi C., 2012 − Effect of dietary lipid and protein levels with different protein to energy ratios on growth performance, feed utilization and body composition of Rutilus frisii kutum (Kamenskii, 1901) fingerlings, Aquaculture Research.

10. Esterbauer H., Schaur R. J. and Zollner H., 1991 − Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes, Free Radical Biology and Medicine, 11, 81-128.

11. Gatlin D., Poe W. E. and Wilson R. P., 1986 − Effects of singular and combined dietary deficiencies of selenium and vitamin E on fingerling channel catfish (Ictalurus punctatus), The Journal of Nutrition, 116, 1061-1067.

12. Halver J. E. and Hardy R. W., 2002 − Nutrient flow and retention, Fish nutrition, 3, 755-770.

13. Hamre K., 2011 − Metabolism, interactions, requirements and functions of vitamin E in fish, Aquaculture Nutrition, 17, 98-115.

14. Heyrati F. P., Mostafavi H., Toloee H. and Dorafshan S., 2007 − Induced spawning of kutum, Rutilus frisii kutum (Kamenskii, 1901) using (D-Ala 6, Pro 9-NEt) GnRHa combined with domperidone, Aquaculture, 265, 288-293.

15. Huang C.-H. and Huang S.-L., 2004 − Effect of dietary vitamin E on growth, tissue lipid peroxidation, and liver glutathione level of juvenile hybrid tilapia, (Oreochromis niloticus) × (O. aureus), fed oxidized oil, Aquaculture, 237, 381-389.

16. Kei S., 1978 − Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method, Clinica Chimica Acta, 90, 37-43.

17. Köhrle J., 2004 − Selenium in biology and medicine − further progress and increasing interest, Journal of Trace Elements in Medicine and Biology, 18, 61-63.

18. Mittler R., 2002 − Oxidative stress, antioxidants and stress tolerance, Trends in plant science, 7, 405-410.

19. NRC, 1993 − Nutrient requirements of fish (NRC), Washington, DC: National Academic Press.

20. Ouraji H., Khalili K. J., Ebrahimi G. and Jafarpour S. A., 2011 − Determination of the optimum transfer time of kutum (Rutilus frisii kutum) larvae from live food to artificial dry feed, Aquaculture International, 19, 683-691.

21. Özkan-Yılmaz F., Özlüer-Hunt A., Gündüz S. G., Berköz M. and Yalın S., 2013 − Effects of dietary selenium of organic form against lead toxicity on the antioxidant system in Cyprinus carpio, Fish physiology and biochemistry, 1-9.

22. Parker R. and Parker R., 2011 − Aquaculture science, CengageBrain.com.

23. Peng S., Chen L., Qin J., Hou J., Yu N., Long Z., Li E. and Ye J., 2009 − Effects of dietary vitamin E supplementation on growth performance, lipid peroxidation and tissue fatty acid composition of black sea bream (Acanthopagrus schlegeli) fed oxidized fish oil, Aquaculture Nutrition, 15, 329-337.

24. Peterson G. L., 1977 – A simplification of the protein assay method of Lowry et al. which is more generally applicable, Analytical biochemistry, 83, 346-356.

25. Poston H. A., Combs Jr. G. F. and Leibovitz L., 1976 − Vitamin E and selenium interrelations in the diet of Atlantic salmon (Salmo salar): gross, histological and biochemical deficiency signs, The Journal of Nutrition, 106, 892.

26. Ribeiro A., Ribeiro L., Sæle Ø., Hamre K., Dinis M. and Moren M., 2012 − Selenium supplementation changes glutathione peroxidase activity and thyroid hormone production in Senegalese sole (Solea senegalensis) larvae, Aquaculture Nutrition, 18, 559-567.

27. Rider S. A., Davies S. J., Jha A. N., Fisher A. A., Knight J. and Sweetman J. W., 2009 − Supranutritional dietary intake of selenite and selenium yeast in normal and stressed rainbow trout (Oncorhynchus mykiss): Implications on selenium status and health responses, Aquaculture, 295, 282-291.

28. Rotruck J., Pope A., Ganther H., Swanson A., Hafeman D. G. and Hoekstra W., 1973 − Selenium: biochemical role as a component of glutathione peroxidase, Science, 179, 588-590.

29. Wang H., Zhang J. and Yu H., 2007a − Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice, Free Radical Biology and Medicine, 42, 1524-1533.

30. Wang Y., Han J., Li W. and Xu Z., 2007b − Effect of different selenium source on growth performances, glutathione peroxidase activities, muscle composition and selenium concentration of allogynogenetic crucian carp (Carassius auratus gibelio), Animal feed science and technology, 134, 243-251.

31. Watanabe T., Kiron V. and Satoh S., 1997 − Trace minerals in fish nutrition, Aquaculture, 151, 185-207.

32. Wise D., Tomasso J., Gatlin D., Bai S. and Blazer V., 1993 − Effects of dietary selenium and vitamin E on red blood cell peroxidation, glutathione peroxidase activity, and macrophage superoxide anion production in channel catfish, Journal of Aquatic Animal Health, 5, 177-182.

33. Zhou X., Wang Y., Gu Q. and Li W., 2009 − Effects of different dietary selenium sources (selenium nanoparticle and selenomethionine) on growth performance, muscle composition and glutathione peroxidase enzyme activity of crucian carp (Carassius auratus gibelio), Aquaculture, 291, 78-81.

34. Zhu Y., Chen Y., Liu Y., Yang H., Liang G. and Tian L., 2012 − Effect of dietary selenium level on growth performance, body composition and hepatic glutathione peroxidase activities of largemouth bass Micropterus salmoide, Aquaculture Research, 43, 1660-1668.

Transylvanian Review of Systematical and Ecological Research

The Journal of „Lucian Blaga“ University of Sibiu

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