Nanoparticles Ecotoxicity on Daphnia magna

Open access


In recent years, development of nanotechnology as well as the toxicity potential of nanomaterials on the environment has received much attention. In order to assess the potential toxic impact of nanoparticles on aquatic environments, we used three kinds of nanoparticles, including titanium dioxide (TiO2), copper oxide (CuO), and zinc oxide (ZnO) on an aquatic model species, Daphnia magna. In fact, Daphnia magna was exposed to different concentrations for 24, 48, 72, and 96 h at 20-25°C. All the important water quality parameters, such as temperature, pH, and dissolved oxygen (DO) were controlled to meet the standard requirements during the experiment. The LC50 values for 24, 48, 72, and 96 h were estimated statistically using Probit methods. The LC50 48 h values for TiO2, CuO, and ZnO were 171.88 mg/l, 6.62 mg/l, and 3.23 mg/l, respectively.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Adam N. Alexander V. Dries K. and Ronny B. 2015 ‒ The chronic toxicity of CuO nanoparticles and copper salt to Daphnia magna Journal of Hazardous Materials 283 416-422 doi:

  • 2. Ates M. Daniels J. Arslan Z. and Farah I. O. 2013 ‒ Effects of aqueous suspensions of titanium dioxide nanoparticles on Artemia salina: assessment of nanoparticle aggregation accumulation and toxicity Environmental Monitoring Assessessment 185 4 3339-48 doi: 10.1007/s10661-012-2794-7.

  • 3. Baek Y. W. and An Y. J. 2011 ‒ Microbial toxicity of metal oxide nanoparticles (CuO NiO ZnO and Sb2O3) to Escherichia coli Bacillus subtilis and Streptococcus aureus Science of The Total Environment 409 8 1603-8 doi: 10.1016/j.scitotenv.2011.01.014.

  • 4. Basak P. K. and Konar S. K. 1977 ‒ Estimation of Safe Concentration of Insecticides A New Method Tested on DDT and BHC Journal of Inland Fisher Society 9 19-29.

  • 5. Blinova I. Ivask A. Heinlaan M. Mortimer M. and Kahru A. 2010 ‒ Ecotoxicity of nanoparticles of CuO and ZnO in natural water Environmental Pollution 158 1 41-7 doi: 10.1016/j.envpol.2009.08.017.

  • 6. Brunner T. J. Wick P. Manser P. Spohn P. Grass R. N. Limbach L. K. Bruinink A. and Stark W. J. 2006 – In vitro cytotoxicity of oxide nanoparticles: comparison to asbestos silica and the effect of particle solubility Environmental Science and Technology 40 14 4374-81.

  • 7. Fabrega J. Luoma S. N. Tyler C. R. Galloway T. S. and Lead J. R. 2013 – Silver nanoparticles: behaviour and effects in the aquatic environment Environmental International 37 2 517-31 doi: 10.1016/j.envint.2010.10.012.

  • 8. Griffitt R. J. Luo J. Gao J. Bonzongo J. C. and Barber D. S. 2008 – Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms Environmental Toxicology and Chemistry 27 9 1972-8 doi: 10.1897/08-002.1.

  • 9. Hai Z. Zhao Z. Lu G. H. Xia J. and Jin S. 2012 – Toxicity of nanoscale CuO and ZnO to Daphnia magna Chemical Research in Chinese Universities 28 2 209-213.

  • 10. Heinlaan M. Angela I. Irina B. Henri-Charles D. and Anne K. 2008 – Toxicity of nanosized and bulk ZnO CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus Chemosphere 71 7 1308-1316 doi:

  • 11. Jaafarzadeh N. Hashempour Y. and Ahmadi Angali K. 2013 – Acute toxicity test using cyanide on Daphnia magna by flow-through system Journal of Water Chemistry and Technology 35 6 281-286 doi: 10.3103/s1063455x13060076.

  • 12. Johari S. A. and Asghari S. 2015 – Acute toxicity of titanium dioxide nanoparticles in Daphnia magna and Pontogammarus maeoticus Journal of Advances in Environmental Health Research 3 2 111-119.

  • 13. Khoshnood Z. Jamili S. Khodabandeh S. Mashinchian Moradi A. and Motallebi Moghanjoghi A. A. 2014 – Histopathological effects and toxicity of atrazine herbicide in Caspian Kutum Rutilus frisii kutum fry Iranian Journal of Fisheries Sciences 13 3 702-718.

  • 14. Liu J. Deling F. Lei W. Lili S. Jie D. Yingwen C. and Shubao S. 2014 – Effects of Zno Cuo Au and Tio2 Nanoparticles on Daphnia magna And Early Life Stages Of Zebrafish Danio rerio Environment Protection Engineering 40 1 139-149 doi: 10.5277/epe140111.

  • 15. Lopes S. Ribeiro F. Wojnarowicz J. Lojkowski W. Jurkschat K. Crossley A. Soares A. M. and Loureiro S. 2014 – Zinc oxide nanoparticles toxicity to Daphnia magna: size-dependent effects and dissolution Environmental Toxicology and Chemistry 33 1 190-8 doi: 10.1002/etc.2413.

  • 16. Lovern S. B. and Klaper R. 2006 – Daphnia magna mortality when exposed to titanium dioxide and fullerene (C60) Environmental Toxicology and Chemistry 25 4 1132-7.

  • 17. Masciangioli T. and Wei-Xian Z. 2003 – Peer Reviewed: Environmental Technologies at the Nanoscale Environmental Science and Technology 37 5 102A-108A doi: 10.1021/es0323998.

  • 18. McMahon J. W. and Rigler F. H. 1965 – Feeding Rate of Daphnia Magna Straus in Different Foods Labeled with Radioactive Phosphorus Limnology and Oceanography 10 1 105-113 doi: 10.4319/lo.1965.10.1.0105.

  • 19. OECD. 2004 – Guideline for the testing of chemicals Organisation for Economic Co-operation and Development (OECD).

  • 20. Ozkan Y. Ilhan A. Hasan I. and Munever S. 2015 – Determination of TiO2 and AgTiO2 Nanoparticles in Artemia salina: Toxicity Morphological Changes Uptake and Depuration Bulletin of Environmental Contamination and Toxicology 96 1 36-42 doi: 10.1007/s00128-015-1634-1.

  • 21. Ranjbar M. JaafarZadeh N. Piri M. and Khodadady M. 2011 – Short communication-Survey of Methyl Tertiary Butyl Ether (MTBE) toxicity using bioassay on Daphnia magna Iranian Journal of Fishery Science 10 3 541-545.

  • 22. Strigul N. Liana V. Catherine G. Mahmoud W. Xuyang L. Christos C. and Kristin J. 2009 – Acute toxicity of boron titanium dioxide and aluminium nanoparticles to Daphnia magna and Vibrio fischeri Desalination 248 1-3 771-782 doi:

  • 23. Wang D. Zhifen L. Ting W. Zhifeng Y. Mengnan Q. Shourong Z. and Wei L. 2016 – Where does the toxicity of metal oxide nanoparticles come from: The nanoparticles the ions or a combination of both? Journal of Hazardous Materials 308 328-334 doi:

  • 24. Wiench K. Wendel W. Volker H. Kristin R. Edward S. Sabine Z. and Robert L. 2009 – Acute and chronic effects of nano- and non-nano-scale TiO2 and ZnO particles on mobility and reproduction of the freshwater invertebrate Daphnia magna Chemosphere 76 10 1356-1365 doi:

  • 25. Zhu X. Chang Y. and Chen Y. 2010 – Toxicity and bioaccumulation of TiO2 nanoparticle aggregates in Daphnia magna Chemosphere 78 3 209-15 doi: 10.1016/j.chemosphere.2009.11.013.

  • 26. Zhu X. Lin Z. Yongsheng C. and Shengyan T. 2008 – Acute toxicities of six manufactured nanomaterial suspensions to Daphnia magna Journal of Nanoparticle Research 11 1 67-75 doi: 10.1007/s11051-008-9426-8.

Journal information
Cited By
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 475 189 7
PDF Downloads 204 105 4