The action framework at the European Union level for the conservation of biodiversity was set up based on the Birds Directive (79/409/EEC) and Habitats Directive (92/43/EEC). One principal element of the implementation of these two significant Directives in Croatia is the set up of a Natura 2000 network of protected areas, a network which should be based on a specific monitoring plan at Croatian national level for each species which is considered of community interest. In this general context, this study suggests a set of monitoring elements for Rhodeus sericeus amarus for the Croatian Continental Biogeographical Region. The suggestions are based on eight selected criteria: Croatian national borders proximity sectors overlay; very good quality populations of Rhodeus sericeus amarus in terms of population density and structure (e.g. protected areas) in characteristic good habitats; habitats which need ecological reconstruction to allow this fish species populations structure ameliorate or natural repopulation; key sectors with importance for connectivity (e.g. lotic sectors between different important sectors, rivers confluence areas, etc.); sectors influenced by human impact like: industrial pollution point sources, sectors influenced by agricultural diffuse sources of pollution, sectors influenced by habitats modifications (watercourses remodeling, watercourses regulation, etc.), geographically extreme monitoring sections in the most-upstream and mostdownstream sections of the rivers, in this species range and in the near outer proximities of these extremes