Objective: To observe the effects of electro-acupuncture (EA) on ultrastructure of gastric antrum smooth muscle cells (GASMCs) in diabetic gastroparesis (DGP) model rats, and to explore the possible mechanism underlying the therapeutic effects of EA.
Methods: Sixty Sprague Dawley rats were randomly divided into 5 groups: blank control (group A), DGP model (group B), EA point (group C), EA non-point (group D), Metoclopramide control (group E). DGP rat model was established by 2% STZ intraperitoneal injection at once, and then being fed with high-sugar-high-fat (HSHF) for 8wks. Phenol red lavage method was employed to measure gastric emptying rate (GER) and intestinal migration rate (IMR). The ultrastructure of GASMCs was observed under the electronic microscope.
Results: Compared with the blank control group, the GER and IMR in model group were decreased significantly (P＜0.05 or P＜0.01). Compared with the model group, the GER and IMR in EA point group were increased significantly(P＜0.05 or P＜0.01). Electro-acupuncture on “Zu San Li”(ST36) point, etc. has been observed improving the ultrastructure of GASMCs, as well as increasing the number of interstitial cells of Cajal (ICCs).
Conclusion: Electro-acupuncture can improve the GI motility promisingly, based on a potential underlying mechanism that the electro-acupuncture can improve the ultrastructure of gastric smooth muscle, and increase the number of ICCs.
1 Rong-Qin JIANG. Research progress on diabetic gastroparesis: its etiology, pathogenesis, diagnosis and treatment. Chinese Journal of Clinical Medicine2009;37(11):43-45.
2 Quan-Quan WAN, Feng-E HE, Ya-Ping LIN. Observation on indexes related to the evolution process of diabetic gastroparesis rat models. Journal of Hunan University of Chinese Medicine 2014;34(10):6-10.
3 Yun GUO, De-Zhi WANG, Qiang ZHOU. Overview of combined Chinese and western medicines on diabetic gastroparesis. Shandong Journal of Traditional Chinese Medicine 2012；31(3)：221-223.
4 Yi ZHONG, Zi-Ming ZHAO, Yin-Jie LU, et al. Effects of Wei Chang Shu on smooth muscle cells of gastric antrum in diabetic gastroparesis rats. Chinese Journal of Experimental Traditional Medical Formulae 2009;15(9):71-77.
5 Qiu-Hai QIAN, Zhi-Cheng WANG, Jian-Ping WEI, et al. Influence of Tangweian granule on the ultrastructure in rat model with diabetic gastroparesis. Chinese Journal of Clinical Pharmocology 2007;23(4):311-312.
6 Ya-Ping ZHANG, Huan-Xue ZHANG, Jin-Yan LUO, et al. Study on the ultrastructural changes of interstitial cells of Cajal in diabetic gastroparesis rats. Chinese Journal of Digestion 2003;23(2):116-117.